Overview
- Editors:
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Melody S. Clark
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Molecular Genetics Unit, School of Clinical Medicine, Department of Medicine, Level 5, Addenbrooke’s Hospital, University of Cambridge, Cambridge, UK
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Table of contents (13 chapters)
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Front Matter
Pages I-XXIX
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Basic Molecular Techniques
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- S. Wilkie, M. S. Clark, P. Leroy, M. Merlino, S. Nègre, J. C. Caissard et al.
Pages 3-53
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- A. Guevara-Garcia, L. Herrera-Estrella, G. Olmedo-Alvarez, M. S. Clark, G. Elgar, C.-N. Liu et al.
Pages 54-153
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- P. Lessard, V. Decroocq, M. Thomas, H. C. C. Foote, G. Brady, G. J. Thorlby et al.
Pages 154-220
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Characterization of Plant DNA
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Front Matter
Pages 279-279
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- M. Landgren, K. Glimelius
Pages 281-304
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- J. H. van den Berg, S. D. Chasalow, R. Waugh
Pages 334-396
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Genetic Engineering: Methodology and Analysis
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Front Matter
Pages 397-397
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- R. H. Potter, M. G. K. Jones
Pages 399-426
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- J. F. Topping, K. Lindsey
Pages 427-442
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- I. J. Leitch, A. Y. Kenton, A. S. Parokonny, M. D. Bennett
Pages 461-485
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- A. Y. Kenton, A. S. Parokonny, I. J. Leitch, M. D. Bennett
Pages 486-511
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Back Matter
Pages 521-529
About this book
The aim of this manual is to encompass a broad range of the latest plant molecular biology techniques. However, it is acknowledged that any manual will be read (and hopefully) used by a wide range of people with different levels of experience. Hence the remit of the manual was widened to include a full range of basic molecular tech niques, so that novices do not have to consult several texts to enable the execution of each major experiment. The manual is divided into three main parts: Part I: Basic Molecular Techniques The raison d'etre behind this part is to provide a background knowledge of molecular techniques, but also to reduce duplication in later chapters (this is particularly true of the methods contained in Chap. 1). All authors provided very detailed methods and often forgot that so me of these would be covered earlier. A particular favourite was DNA extraction methods, wherc everyone managed to provide a slightly different variant! My view was that it is far less confusing for the reader to be presented with one standard protocol and accom panying troubleshooting tips, than to read a different version in each chapter. In this way the basic techniques are addressed more in depth (and my apologies to all authors for judicious use of the delete key!). RNA methodology is covered in Chapter 3. This proceeds from the fundamentals of extraction, northern blotting etc. , to cDNA libraries.
Reviews
"Overall, this manual deserves to be highly recommended due, in particular, to the clarity of writing. ...this manual will be purchased and used by many in this rapidly expanding area of research." Heredity
"...presents a broad range of techniques needed by plant molecular biologists. The protocols are clearly presented and referenced." The New Phytologist
Editors and Affiliations
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Molecular Genetics Unit, School of Clinical Medicine, Department of Medicine, Level 5, Addenbrooke’s Hospital, University of Cambridge, Cambridge, UK
Melody S. Clark
