Skip to main content
SpringerLink
Log in
Book cover

Fingerprinting Methods Based on Arbitrarily Primed PCR

  • Book
  • © 1997

Overview

Editors:
  1. Maria Rita Micheli

    1. Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Perugia, Italia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Rodolfo Bova

    1. Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma “Tor Vergata”, Roma, Italia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Lab Manuals (SLM)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (41 chapters)

  1. Front Matter

    Pages I-XVI
    Download chapter PDF

  2. DNA Fingerprinting

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Overview

      • Maria Rita Micheli, Rodolfo Bova
      Pages 3-13

    3. DNA Extraction

      1. DNA Extraction from Mammals
        • E. D’Ambrosio, E. Pascale
        Pages 15-20
      2. Insect DNA Extraction Protocol
        • G. J. Hunt
        Pages 21-24
      3. Rapid DNA Extraction from Plants
        • C. Neal Stewart Jr.
        Pages 25-28
      4. Preparation of Fungal Genomic DNA for PCR and RAPD Analysis
        • G. C. Graham, R. J. Henry
        Pages 29-34
      5. Extraction of <i>Histoplasma capsulatum</i> DNA for PCR
        • J. P. Woods, D. E. Berg, K. L. Fisher
        Pages 35-40
      6. DNA Extraction from Bacterial Cultures
        • M. Bazzicalupo, S. Fancelli
        Pages 41-45

    4. Fingerprint Production

      1. Arbitrarily Primed PCR and RAPDs
        • R. Arribas, S. Tòrtola, J. Welsh, M. McClelland, M. A. Peinado
        Pages 47-53
      2. Random Amplified Polymorphic DNA Assay
        • M. R. Micheli, R. Bova, E. D’Ambrosio
        Pages 55-63
      3. DNA Amplification Fingerprinting
        • G. Caetano-Anollés
        Pages 65-80
      4. Fluorescent Detection and Analysis of RAPD Amplicons Using the ABI PRISM DNA Sequencers
        • J. Walsh Weller, A. Reddy
        Pages 81-92
      5. Optimization of RAPD Fingerprinting
        • B. Cobb
        Pages 93-102
      6. Fingerprint Tailoring
        • G. Caetano-Anollés
        Pages 103-117

    6. Marker Isolation and Characterization

      1. Recovering Amplified DNA from Silver Stained Gels
        • G. Caetano-Anollés
        Pages 153-160
Back to top

Keywords

About this book

DNA and RNA fingerprinting based on arbitrarily primed PCR provides the most powerful tool for the study of genes.
The basic techniques are described in detailed protocols including each step from template preparation to fingerprint visualization. Various protocols for the basic techniques allow to choose between alternative strategies. In addition to the general techniques specific research applications of particular interest are given such as gene mapping, detection of somatic mutations, gene abnormally expressed in tumors or differentially expressed genes by RNA fingerprinting.

Reviews

"The only primer you'll ever need... great care has been taken in putting this book together, and the results are very impressive. Although the work of many authors, the contributions form a coherent and well-organized whole." Trends in Genetics
"...buy this excellent book." Cellular and Molecular Biology

Editors and Affiliations

  • Dipartimento di Biologia Cellulare e Molecolare, Università di Perugia, Perugia, Italia

    Maria Rita Micheli

  • Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Roma “Tor Vergata”, Roma, Italia

    Rodolfo Bova

Bibliographic Information

  • Book Title: Fingerprinting Methods Based on Arbitrarily Primed PCR

  • Editors: Maria Rita Micheli, Rodolfo Bova

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-60441-6

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1997

  • Softcover ISBN: 978-3-642-47812-3Published: 23 August 2014

  • eBook ISBN: 978-3-642-60441-6Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: XVI, 441

  • Topics: Cell Biology, Molecular Medicine, Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation