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Over 40 brillant colour photos showing the results of fluorescence in situ hybridization on various tissues
Comprehensive collection of proven lab protocols, each provided by an expert in the field
Detailed step-by-step protocols, including troubleshooting sections and practical comments
Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.
Content Level »Professional/practitioner
Keywords »Chromosom - DNA - FISH - Fluoreszenz in situ-Hybridisierung - Interphase - PCR - Telomere - chromosome - genetics - genomic alterations - hybridization - in situ hybridization - microscopy - tissue - translation
List of Contents.- The Standard FISH Procedure - an Introduction.- 1 FISH Techniques, FISH Probes and Their Applications in Medicine and Biology - An Overview.- 2 Microscopy and Imaging Systems.- FISH on Native, Human Tissues.- 3 FISH on Chromosome Preparations of Peripheral Blood.- 4 FISH on Uncultured Blood or Bone Marrow.- 5 FISH on Blood or Bone Marrow Smears.- 6 Epithelial Cells from Buccal Smears and Urine.- 7 FISH on Hair Root Cells.- 8 Amniotic Fluid Cells - Uncultured.- 9 Human Sperm Cells.- FISH on Archival, Human Tissues.- 10 Archival Cytogenetic Slides.- 11 Formalin-Fixed and Paraffin-Embedded Tissue Sections.- 12 Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue.- 13 Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue.- 14 Nucleus Extraction from Cryofixed Tissue.- 15 Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells.- 16 Comparative Genomic Hybridization.- Special FISH Techniques (Fiber-FISH, PNA-FISH, Co-FISH, NU-FISH).- 17 High Resolution FISH of Stretched Chromosome Fibers.- 18 Quantitative DNA Fiber Mapping.- 19 PNA-FISH.- 20 Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH.- 21 The Combined Use of Nuclease in Situ Digestion and FISH (NU-FISH).- 22 DNA Breakage Detection-FISH (DBD-FISH).- 23 Detection of Radiosensitivity by Means of Three-Color FISH.- 24 Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T.- FISH in Zoology and Virology.- 25 Zoo-FISH.- 26 FISH on Insect Cells Transfected with Heterologous DNA.- 27 Yeast FISH: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells.- 28 In Situ Detection of Viral Nucleic Acids by Chemiluminescence.- Multicolor FISH Applications.- 29 Spectral Imaging Analysis of Metaphase and Interphase Cells.- 30 Microdissection of Chromosomes and Reverse FISH.- 31 Multicolor FISH Techniques for the Detection of Inter- and Intrachromosomal Rearrangements.- 32 Centromere-Specific Multicolor-FISH (cenM-FISH).- 33 Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material.- 34 Simultaneous Fluorescence Immunophenotyping and FISH (FICTION).- Not FISH but Almost.- 35 PRINS: Hybridization and Primed in Situ Labeling in One Step.- 36 Microarrays and DNA-Chips in the Analysis of Genetic Abberrations.