Fluorescence In Situ Hybridization (FISH) - Application Guide

1. Front Matter

   Pages i-xviii

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2. Molecular Cytogenetics, Probes and Equipment

   1. Molecular Cytogenetic Applications in Diagnostics and Research: An Overview

      • Nedime Serakinci, Steen Kølvraa

      Pages 3-21

   2. Molecular Cytogenetics: The Standard FISH and PRINS Procedure

      • Thomas Liehr, Franck Pellestor

      Pages 23-34

   3. Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes

      • Fengtang Yang, Vladimir Trifonov, Bee Ling Ng, Nadezda Kosyakova, Nigel P. Carter

      Pages 35-52

   4. ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results

      • Anja Weise, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Madeleine Gross, Vivien Klaschka et al.

      Pages 53-60

   5. PNA-FISH Technique for In Situ Assessment of Aneuploidy

      • Franck Pellestor, Petra Paulasova, Samir Hamamah

      Pages 61-66

   6. Oligonucleotide FISH Probes

      • Sean Bradley, Leah Zamechek, Joan Aurich-Costa

      Pages 67-73

   7. Microscopy and Imaging Systems

      • Ivan Y. Iourov

      Pages 75-84

   8. Optical Filters for FISH

      • Michael Sommerauer, Ingrid Feuerbacher

      Pages 85-97

   9. FISH With and Without COT1 DNA

      • Vladimir A. Trifonov, Nadezhda N. Vorobieva, Willem Rens

      Pages 99-109

3. FISH Applications in Different Tissue Preparations

   1. Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblasts

      • Anja Weise, Thomas Liehr

      Pages 113-122

   2. Prenatal Diagnostics on Uncultured Amniocytes

      • Bernd Eiben, Ralf Glaubitz, Arens Hans-Jürgen

      Pages 123-130

   3. Tumorcytogenetic Diagnostics and Research on Uncultured Blood or Bone Marrow and Smears

      • Anita Heller, Madeleine Gross, Vera Witthuhn, Katja Behr, Sophie Hinreiner, Hasmik Mkrtchyan et al.

      Pages 131-136

   4. Application of FISH to Archival Cytogenetic Slides

      • Thomas Liehr, Erich Gebhart

      Pages 137-140

   5. Characterization of Mosaicism in Various Easy-to-Acquire Body Tissues Such as Buccal Smears, Hair Root Cells or Urine

      • Kristin Mrasek, Anja Weise, Madeleine Gross, Hasmik Mkrtchyan, Thomas Liehr

      Pages 141-146

   6. Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction

      • Thomas Liehr, Marina Manvelyan

      Pages 147-156

   7. FISH on Sperms, Spermatocytes and Oocytes

      • Maria Oliver-Bonet

      Pages 157-172

4. Characterization of Marker Chromosomes

   1. Multiplex FISH and Spectral Karyotyping

      • Thomas Liehr, Kristin Mrasek, Nadezda Kosyakova, Hasmik Mkrtchyan, Joana Melo, Anna Polityko et al.

      Pages 175-181

   2. Tips and Tricks for mFISH

      • Karin M. Greulich-Bode, Maren Kaufmann, Klaus Gilbertz, Roderick A. F. MacLeod

      Pages 183-192

   3. Simultaneous Fluorescence Immunostaining and FISH

      • Christine J. Ye, Lesley Lawrenson, Guo Liu, Joshua Stevens, Steve Bremer, Karen J. Ye et al.

      Pages 193-216

This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

• Chromosom
• Comparative genomic hybridization
• Cytogenetics
• DNA
• Epigenetic aberrations
• Fluoreszenz in situ-Hybridisierung
• Interphase
• Nuclear architecture
• PRINS
• Termination
• bone
• genetics
• hybridization
• microscopy
• tissue

From the review of the previous edition "FISH Technology - Springer Lab Manual", edited by B. Rautenstrauß and T. Liehr

"It is a book that would complement the library of any lab performing FISH experiments and would bring new technologies to labs already scaling the heights or to labs setting out on their journey. … One of the strengths of this book is the description of FISH techniques that have been developed to answer more specific questions about DNA structure and behaviour. … My PhD student has recently been using this book and praised it without knowing I was reviewing it – what better endorsement could there be?" (Joanna M. Bridger, Cell and Chromosome Biology Group, Brunel University, Middlesex, UK, Journal of Cell Science 116, 2375-2376 © 2003 The Company of Biologists Ltd doi:10.1242/jcs.00594)

"Ever wondered how to catch your FISH … in the dark pools of cellular biology? Then … FISH technology might be a welcome addition to your library.Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) … . Every chapter is illustrated by several schematic drawings … . The manual is completed by a subject index … . The book is certainly clear … providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

• Institut für Humangenetik und Anthropologie, Jena, Germany

  Thomas Liehr

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