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Resin Microscopy and On-Section Immunocytochemistry

  • Book
  • © 2001

Overview

Authors:
  1. Geoffrey R. Newman

    1. Medical Microscopy Sciences, University of Wales College of Medicine, Cardiff, UK

    View author publications

    You can also search for this author in PubMed   Google Scholar

  2. Jan A. Hobot

    1. Medical Microscopy Sciences, University of Wales College of Medicine, Cardiff, UK

    View author publications

    You can also search for this author in PubMed   Google Scholar

  • Ready-to-go protocols
  • Explains all steps from fixation to labelling and detection
  • Helps in finding the resin of choice for your needs

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (10 chapters)

  1. Front Matter

    Pages I-XIX
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  2. Resin Embedding

    1. Front Matter

      Pages 1-1
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    2. The Strategic Approach

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 3-33

    3. The Resins

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 34-63

    4. Resin Embedding Protocols for Chemically Fixed Tissue

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 64-87

    5. Cryotechniques

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 88-108

    6. Methods for Resin Polymerisation

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 109-129

    7. Handling Resin Blocks

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 130-134

  3. On-Section Immunolabelling

    1. Front Matter

      Pages 135-135
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    2. Strategies in Immunolabelling

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 137-163

    3. General Considerations

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 164-194

    4. Immunolabelling Protocols for Resin Sections

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 195-214

    5. Resin Embedding and Immunolabelling

      • Geoffrey R. Newman, Jan A. Hobot
      Pages 215-228

  4. Back Matter

    Pages 229-273
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Keywords

About this book

Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.

Reviews

From the reviews of the second edition:

"This comprehensive and valuable book is a must-have for the immunohistochemical laboratory. It extensively covers the field of onsection immunocytochemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling protocols … . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. … it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocytochemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)

Authors and Affiliations

  • Medical Microscopy Sciences, University of Wales College of Medicine, Cardiff, UK

    Geoffrey R. Newman, Jan A. Hobot

Bibliographic Information

  • Book Title: Resin Microscopy and On-Section Immunocytochemistry

  • Authors: Geoffrey R. Newman, Jan A. Hobot

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-56930-2

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2001

  • Hardcover ISBN: 978-3-540-67277-7Published: 10 April 2001

  • Softcover ISBN: 978-3-642-47731-7Published: 23 August 2014

  • eBook ISBN: 978-3-642-56930-2Published: 06 December 2012

  • Edition Number: 2

  • Number of Pages: XIX, 273

  • Topics: Cell Biology, Immunology, Biochemistry, general

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