Immobilized Cells

1. Front Matter

   Pages I-VIII

   PDF

2. Introduction

   1. Characterization of Immobilized Cells; Introduction

      • René H. Wijffels

      Pages 1-5

3. Immobilization

   1. Description of the Support Material

      • Emily J. T. M. Leenen

      Pages 6-14

   2. Description of the Immobilisation Procedures

      • Denis Poncelet, Claire Dulieu, Muriel Jacquot

      Pages 15-30

   3. Measurement of Density, Particle Size and Shape of Support

      • Erik Van Zessen, Johannes Tramper, Arjen Rinzema

      Pages 31-35

   4. Mechanical Stability of the Support

      • Emily J. T. M. Leenen

      Pages 36-43

   5. Diffusion Coefficients of Metabolites

      • Evelien E. Beuling

      Pages 44-64

4. Kinetics

   1. Quantity of Biomass Immobilized, Determination of Biomass Concentration

      • Ellen A. Meijer, René H. Wijffels

      Pages 65-73

   2. Kinetics of the Suspended Cells

      • René H. Wijffels

      Pages 74-76

   3. Diffusion Limitation

      • René H. Wijffels

      Pages 77-84

   4. Micro-Electrodes

      • Dirk De Beer

      Pages 85-100

   5. Biomass Gradients

      • René H. Wijffels

      Pages 101-122

   6. NMR and Immobilized Cells

      • Jean-Noël Barbotin, Jean-Charles Portais, Paula M. Alves, Helena Santos

      Pages 123-138

5. Engineering

   1. Immobilization at Large Scale by Dispersion

      • Denis Poncelet, Stephane Desobry, Ulrich Jahnz, K. Vorlop

      Pages 139-149

   2. Immobilization at Large Scale with the Resonance Nozzle Technique

      • Jan H. Hunik

      Pages 150-161

   3. External Mass Transfer

      • René H. Wijffels

      Pages 162-174

   4. Liquid Fluidization of Gel-Bead Particles

      • Erik Van Zessen, Johannes Tramper, Arjen Rinzema

      Pages 175-181

   5. Gradients in Liquid, Gas or Solid Fractions

      • René H. Wijffels

      Pages 182-190

   6. Support Material Stability at the Process Conditions Used

      • Emily J. T. M. Leenen

      Pages 191-198

6. Cases

   1. Immobilized Cells in Food Technology: Storage Stability and Sensitivity to Contamination

      • Claude P. Champagne

      Pages 199-212

used. Generally in the development of a process, research activity will start with the physiology of the immobilized cells and be concluded by scale-up and application. Guidelines for the different phases in research are given below. Springer Lab Manual about immobilized cells The Springer Lab Manual about immobilized cells is structured in such a way that guidelines that resulted from the conference 'Immobilized Cells: Basics and Applications' were followed and translated into some practical methods. As for the guidelines for immobilized cells a structure was chosen in which general aspects of immobilization were covered, the kinetics were described and engineering aspects were covered. Besides laboratory meth­ ods some simple calculation procedures have been given in this lab man­ ual. The combination of the practical methods with the calculations pro­ vide a tool for carrying out research with immobilized cells. The lab manual concludes with some recent, promising and practical cases of immobilized cells as examples of applications with potential.

• Bioremediation
• Immobilized cells
• Metabolite
• biomass concentration
• biomass gradients
• gel bead particles
• material stability
• measurement of density
• resonance nozzle technique

• Food and Bioprocess Engineering Group, Wageningen University, Wageningen, The Netherlands

  René H. Wijffels

Policies and ethics