Overview
- Includes experimental approaches to the complexity of the biological functions of RasGTPases
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1120)
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Table of contents (26 protocols)
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Front Matter
Keywords
About this book
Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging and “omics” technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways and ultimately, biological activities and functions of Ras GTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of Ras GTPases.
Editors and Affiliations
Bibliographic Information
Book Title: Ras Signaling
Book Subtitle: Methods and Protocols
Editors: Lorenza Trabalzini, Saverio Francesco Retta
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-791-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-790-7Published: 28 January 2014
Softcover ISBN: 978-1-4939-6054-5Published: 27 August 2016
eBook ISBN: 978-1-62703-791-4Published: 28 January 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 425
Number of Illustrations: 31 b/w illustrations, 39 illustrations in colour
Topics: Cell Biology, Proteomics