Trabalzini, Lorenza, Retta, Saverio Francesco (Eds.)
2014, XII, 425 p. 70 illus., 39 illus. in color.
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Includes experimental approaches to the complexity of the biological functions of RasGTPases
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging, and “omics” technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways, and, ultimately, biological activities and functions of RasGTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of RasGTPases.
Content Level »Professional/practitioner
Keywords »Cell signaling - GTP-binding and hydrolyzing proteins - Model systems - Pathological condition treatment - Ras GTPase superfamily - RasGTPases - Signaling pathways