Editors:
- Presents an overview of standard and more recent methods for the functional analysis of the genetic material
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1094)
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Table of contents (28 protocols)
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Front Matter
About this book
In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.
Keywords
- DNA analysis
- DNA labeling in vivo
- DNA thermophoresis
- DNA-dye binding modes
- FISH
- chromatin analysis
- chromatin imaging by fluorescence
- chromatin staining
- cytosine methylation
- detection of apoptotic DNA
- electron and atomic force microscopy
- hydroxy-methylation
- nuclear dispersion
- sister chromatid exchanges
Editors and Affiliations
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Department of Biology, Autonomous University of Madrid Faculty of Sciences, Madrid, Spain
Juan C. Stockert, Alfonso Blázquez-Castro
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Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain
Jesús Espada
Bibliographic Information
Book Title: Functional Analysis of DNA and Chromatin
Editors: Juan C. Stockert, Jesús Espada, Alfonso Blázquez-Castro
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-706-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, New York 2014
Hardcover ISBN: 978-1-62703-705-1Published: 29 October 2013
Softcover ISBN: 978-1-4939-6048-4Published: 23 August 2016
eBook ISBN: 978-1-62703-706-8Published: 08 July 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 365
Number of Illustrations: 30 b/w illustrations, 44 illustrations in colour
Topics: Cell Biology, Human Genetics