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Life Sciences - Cell Biology | Pheromone Signaling - Methods and Protocols

Pheromone Signaling

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1068

Touhara, Kazushige (Ed.)

2013, XV, 399 p. 74 illus., 30 illus. in color.

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  • Includes cutting-edge methods and protocols in the field of pheromone signaling  
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Providing experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.


Authoritative and practical, Pheromone Signaling: Methods and Protocols details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research.

Content Level » Professional/practitioner

Keywords » Behavioral and endocrine changes - Biochemical analysis - Chemosensory systems - Chromatographic purification - Invertebrate and vertebrate models - Neural response measurement - Pheromone receptors

Related subjects » Cell Biology - Internal Medicine

Table of contents 

Part I: Sampling, Chromatographic Purification, and Chemical Analysis of Pheromone Molecules


1. Female Moth Pheromones

            Tetsu Ando


2. Pheromones in the Fruit Fly

            Jacqueline Chin and Joanne Y. Yew


3. Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry

            Milos V. Novotny and Helena A. Soini


4. Murine Non-Volatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1

            Hiroko Kimoto and Kazushige Touhara


5. Chemical Analysis of Aquatic Pheromones in Fish

            Michael Stewart, Cindy F. Baker, and Peter W. Sorensen


6. Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy

            Xinxing Zhang, Jaime H. Noguez, Yue Zhou, and Rebecca A. Butcher


Part II: Approaches to Characterize Gene and Function of Pheromone Receptors


7. Identification of Chemosensory Receptor Genes from Vertebrate Genomes

            Yoshihito Niimura


8. Functional Assays for Insect Olfactory Receptors in Xenopus Oocytes

            Tatsuro Nakagawa and Kazushige Touhara


9. A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors

            Sandeepa Dey, Senmiao Zhan, and Hiroaki Matsunami


10. Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes

            Tomohiro Ishii


Part III: Methods in Measuring Neural Responses to Pheromones


11. Electro-Antennogram and Single Sensillum Recording in Insect Antennae

            Shannon B. Olsson and Bill S. Hansson


12. Calcium Imaging of Pheromone Responses in the Insect Antennal Lobe

            Susy M. Kim and Jing W. Wang


13. Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons

            Angeldeep Kaur, Sandeepa Dey, and Lisa Stowers


14. Whole Mount Imaging of Responses in Mouse Vomeronasal Neurons

            Pei Sabrina Xu and Timothy E. Holy


15. Calcium Imaging of Vomeronasal Organ Response Using Slice Preparations from Transgenic Mice Expressing G-CaMP2

            C. Ron Yu


16. The Electrovomeronasogram: Field Potential Recordings in the Mouse Vomeronasal Organ

            Trese Leinders-Zufall and Frank Zufall


17. Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations

            Julian P. Meeks and Timothy E. Holy


18. Pheromone-Induced Expression of Immediate Early Genes in the Mouse Vomeronasal Sensory System

            Sachiko Haga-Yamanaka and Kazushige Touhara


Part IV: Protocols in Analyzing Behavioral and Endocrine Changes Elicited by Pheromones


19. Insect Pheromone Behavior: Fruit Fly

            Daisuke Yamamoto, Soh Kohatsu, and Masayuki Koganezawa


20. Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans

            Scott J. Neal, Kyuhyung Kim, and Piali Sengupta


21. Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion)

            Heeun Jang and Cornelia I. Bargmann


22. Behavioral Analysis of Pheromones in Fish

            Peter W. Sorensen


23. Analysis of Male Aggressive and Sexual Behavior in Mice

            Takefumi Kikusui


24. Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice

            Olivier Brock, Julie Bakker, and Michael J. Baum


25. Assessing Postpartum Maternal Care, Alloparental Behavior, and Infanticide in Mice: With Notes on Chemosensory Influences

            Kumi O. Kuroda and Yousuke Tsuneoka


26. Testing Smell When It is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse

            Benoist Schaal, Syrina Al Aïn, and Bruno Patris


27. An Assay for Human Chemosignals

            Idan Frumin and Noam Sobel

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