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Life Sciences - Cell Biology | Membrane Biogenesis - Methods and Protocols

Membrane Biogenesis

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1033

Rapaport, Doron, Herrmann, Johannes M. (Eds.)

2013, XIV, 404 p. 80 illus., 48 illus. in color.

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  • Features numerous techniques to study membrane proteins and membrane lipids as well as their interactions
  • Includes detailed, lab-ready protocols and methodologies
  • Contains expert tips and key implementation advice to ensure successful results

Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.

Content Level » Professional/practitioner

Keywords » Biogenesis - Cellular membranes - Membrane lipids - Membrane proteins - Organelle dynamics - Protein-Lipid interaction

Related subjects » Cell Biology

Table of contents 

Part I: Investigation of Membrane Lipids


1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

            Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger


2. Thin-Layer Chromatography of Phospholipids

            Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg


3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics

            Martina Gsell and Günther Daum


Part II: Analysis of Structure and Topology of Membrane Proteins


4. Using 2D Crystals to Analyze the Structure of Membrane Proteins

            Ian Collinson, Janet Vonck, and Dilem Hizlan


5. Crystallization of Membrane Proteins

            Florian G. Müller and C. Roy D. Lancaster


6. Molecular Dynamics Simulations of Membrane Proteins

            Kristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann


7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins

            Christine T. Schwall and Nathan N. Alder


8. Topology Determination of Untagged Membrane Proteins

            Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner


9. selfassembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses

            Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. Sommer


Part III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes


10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane

            Milit Marom and Abdussalam Azem


11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance

            Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen


12. Peptide Interaction with and Insertion into Membranes

            Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai


13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems

            Joseph D. Unsay and Ana J. García-Sáez


14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding Yeast

            Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo


15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins

            Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel


16. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry

            Carolyn Vargas, Johannes Klingler, and Sandro Keller


Part IV: Membrane Biogenesis and Dynamics


17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast Mitochondria

            Dirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann


18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human Cells

            Johanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann


19. An Assay to Monitor the Membrane Integration of Single-Span Proteins

            Katrin Krumpe and Doron Rapaport


20. Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria

            Daniel Weckbecker and Johannes M. Herrmann


Part V: Functional Analysis of Membrane Proteins


21. Reconstitution of Mitochondrial Presequence Translocase into Proteoliposomes

            Martin van der Laan, Ralf M. Zerbes, and Chris van der Does


22. Single Channel Analysis of Membrane Proteins in Artificial Bilayer Membranes

            Philipp Bartsch, Anke Harsman, and Richard Wagner


23. Quantification of Protein Complexes by Blue Native Electrophoresis

            Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, and Ilka Wittig


24. Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions

            Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew, and Jan-Willem de Gier

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