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Membrane Biogenesis

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Doron Rapaport

    1. Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Johannes M. Herrmann

    1. Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Features numerous techniques to study membrane proteins and membrane lipids as well as their interactions
  • Includes detailed, lab-ready protocols and methodologies
  • Contains expert tips and key implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 1033)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Investigation of Membrane Lipids

    1. Front Matter

      Pages 1-1
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    2. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

      • Cagakan Özbalci, Timo Sachsenheimer, Britta Brügger
      Pages 3-20

    3. Thin-Layer Chromatography of Phospholipids

      • Rania M. Deranieh, Amit S. Joshi, Miriam L. Greenberg
      Pages 21-27

    4. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast Genetics

      • Martina Gsell, Günther Daum
      Pages 29-44

  3. Analysis of Structure and Topology of Membrane Proteins

    1. Front Matter

      Pages 45-45
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    2. Using 2D Crystals to Analyze the Structure of Membrane Proteins

      • Ian Collinson, Janet Vonck, Dilem Hizlan
      Pages 47-65

    3. Crystallization of Membrane Proteins

      • Florian G. Müller, C. Roy D. Lancaster
      Pages 67-83

    4. Molecular Dynamics Simulations of Membrane Proteins

      • Kristyna Pluhackova, Tsjerk A. Wassenaar, Rainer A. Böckmann
      Pages 85-101

    5. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane Proteins

      • Christine T. Schwall, Nathan N. Alder
      Pages 103-120

    6. Topology Determination of Untagged Membrane Proteins

      • Iris Nasie, Sonia Steiner-Mordoch, Shimon Schuldiner
      Pages 121-130

    7. self-assembling GFP: A Versatile Tool for Plant (Membrane) Protein Analyses

      • Katharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, Maik S. Sommer
      Pages 131-144

  4. Studying Protein-Protein and Protein-Lipids Interactions within Membranes

    1. Front Matter

      Pages 145-145
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    2. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial Membrane

      • Milit Marom, Abdussalam Azem
      Pages 147-155

    3. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon Resonance

      • Zht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, Arnold J. M. Driessen
      Pages 157-172

    4. Peptide Interaction with and Insertion into Membranes

      • Ron Saar-Dover, Avraham Ashkenazi, Yechiel Shai
      Pages 173-183

    5. Scanning Fluorescence Correlation Spectroscopy in Model Membrane Systems

      • Joseph D. Unsay, Ana J. García-Sáez
      Pages 185-205

    6. Analyses of Protein–Protein Interactions by In Vivo Photocrosslinking in Budding Yeast

      • Takuya Shiota, Shuh-ichi Nishikawa, Toshiya Endo
      Pages 207-217

    7. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins

      • Aline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, Christine Ebel
      Pages 219-251

    8. Membrane Partitioning and Translocation Studied by Isothermal Titration Calorimetry

      • Carolyn Vargas, Johannes Klingler, Sandro Keller
      Pages 253-271
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Keywords

About this book

Membrane proteins and membrane lipids form complex interactive systems that are highly dynamic and able to be studied only by combinations of different in vivo and in vitro techniques.  In Membrane Biogenesis: Methods and Protocols, experts in the field present a broad collection of methods to study the biogenesis and function of cellular membranes. Beginning with how membrane lipids or membrane proteins can be studied, this detailed volume continues with sections covering different procedures to investigate the interaction of membrane proteins among each other or with membrane lipids, methods to study the biogenesis of membrane proteins and the dynamics of organelles, as well as protocols for the analyses of the functions or complex organization of membrane proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Extensive and easily applicable, Membrane Biogenesis: Methods and Protocols provides readers with a comprehensive but still concise collection including both basic protocols of rather general application and more specialized methods for specific and novel techniques.

Editors and Affiliations

  • Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany

    Doron Rapaport

  • Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany

    Johannes M. Herrmann

Bibliographic Information

  • Book Title: Membrane Biogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Doron Rapaport, Johannes M. Herrmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-487-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-486-9Published: 01 September 2013

  • Softcover ISBN: 978-1-4939-6304-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-487-6Published: 31 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 404

  • Number of Illustrations: 32 b/w illustrations, 48 illustrations in colour

  • Topics: Cell Biology, Membrane Biology

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