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Life Sciences - Cell Biology | The Myc Gene - Methods and Protocols

The Myc Gene

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1012

Soucek, Laura, Sodir, Nicole M. (Eds.)

2013, XI, 282 p. 44 illus., 29 illus. in color.

A product of Humana Press
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  • Presents current up-to-date methodologies
  • Features tips on trouble-shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Content Level » Professional/practitioner

Keywords » Myc - Myc mechanism - cancer - cancer therapy - cellular functions - in vitro and in vivo

Related subjects » Cell Biology - Human Genetics

Table of contents 

1. The Myc World Within Reach

Nicole M. Sodir and Laura Soucek


2. Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs

Marie-Eve Beaulieu, François-Olivier Mc Duff, Mikaël Bédard, Martin Montagne and Pierre Lavigne


3. Analyzing Myc in Cell Transformation and Evolution

Markus Hartl and Klaus Bister


4. Identifying Myc Interactors

Romina Ponzielli, William B. Tu, Igor Jurisica,  and Linda Z. Penn


5. Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation

Colin J. Daniel, Xiaoli Zhang and Rosalie C. Sears


6. An Efficient Way of Studying Protein–Protein Interactions Involving HIF-α, c-Myc, and Sp1

Kenneth K.-W. To and L. Eric Huang


7. Methods for Determining Myc-induced Apoptosis

Dan Lu and Trevor D. Littlewood


8. Methods to Study Myc-regulated Cellular Senescence

Vedrana Tabor, Matteo Bocci and Lars-Gunnar Larsson


9. Chromatin Immunoprecipitation Assays for Myc and N-Myc

Bonnie L. Barrilleaux, Rebecca Cotterman, and Paul S. Knoepfler


10. Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression

Rui Song, Nicole Sponer, and Lin He


11. Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing

Rene Jackstadt, Antje Menssen and Heiko Hermeking


12. A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets

Carla Grandori


13. Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells

John T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, and Davide Ruggero


14. Studying Myc’s Role in Metabolism Regulation

Anne Le and Chi V. Dang


15. Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia

Kavya Rakhra and Dean W. Felsher


16. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis

David J Huels, Patrizia Cammareri, Rachel A Ridgway, Jan P Medema and Owen J Sansom


17. Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS)

Natasa Todorovic-Rakovic


18. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules

Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov

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