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Life Sciences - Cell Biology | Necrosis - Methods and Protocols


Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1004

McCall, Kimberly, Klein, Charles (Eds.)

2013, XI, 252 p. 51 illus., 25 illus. in color.

A product of Humana Press
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  • Includes a wide range of techniques for the study of necrosis in vitro and in vivo
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
Cell death is an essential process in development, and a major contributor to a wide range of human diseases.  Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Necrosis: Methods and Protocols describes techniques  in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

Content Level » Professional/practitioner

Keywords » Biochemical - apoptosis in vivo - apoptotic cell death - autophagic cell death - entosis - mammalian cells - mammalian tissues - necrosis - pyroptosis - signaling pathways

Related subjects » Cell Biology

Table of contents 

Part I: Analysis of Necrosis In Vitro

1. Navigation to the Graveyard- Induction of Various Pathways of Necrosis and their Classification by Flow Cytometry

Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens, Kirsten Lauber, Martin Herrmann

 2. Time-lapse Imaging of Necrosis

Fredrik Wållberg, Tencho Tenev, and Pascal Meier

 3. Activity Assays for Receptor Interacting Protein Kinase 1: A Key Regulator of Necroptosis

Jenny L. Maki and Alexei Degterev

 4. Fluorescent Biosensors for the Detection of HMGB1 Release

Isabelle Martins, Oliver Kepp, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Erika Vacchelli, Lorenzo Galluzzi , and Guido Kroemer

 Part II:  Alternative Cell Death Modalities

5. Methods for the Study of Entosis

Qiang Sun and Michael Overholtzer

 6. Analysis of Pyroptosis in Bacterial Infection

Lia Danelishvili and Luiz E Bermudez

 Part III: Analysis of Cell Death in Mammalian Tissues

7. Analysis of Cell Death by Electron Microscopy

S. Burattini and E. Falcieri

 8. Histological Analysis of Neurodegeneration in the Mouse Brain

Hiroo Yamaguchi and Jie Shen

 9. Quantitation of Acute Necrosis after Experimental Myocardial Infarction

Xin-Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, & Edward B. Thorp

 10. Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin

Marion C. Bonnet

 Part IV: Cell Death in Non-Mammalian Model Systems

 11. Identification and Characterization of Programmed Cell Death Markers in Bacterial Models

Daniel J. Dwyer and Jonathan A. Winkler

 12. Quantification of Genetically Controlled Cell Death in Budding Yeast

 Xinchen Teng and J. Marie Hardwick

 13. Necrosis in C. elegans

Matt Crook, Avni Upadhyay and Wendy Hanna-Rose

 14. Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans

Zao Li, Nan Lu, Xiangwei He, and Zheng Zhou

 15. Modeling Calcium-overload Mediated Necrosis in Drosophila

Kai Liu, Yuhong Li and Lei Liu

 16. Use of Necrotic Markers in the Drosophila Ovary

Allison K. Timmons, Tracy L. Meehan, Tori D. Gartmond, and Kimberly McCall

 17. Detection and Measurement of Necrosis in Plants

Elena A. Minina, Lada H. Filonova, Geoffrey Daniel, and Peter V. Bozhkov

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