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Life Sciences - Cell Biology | Cell Senescence - Methods and Protocols

Cell Senescence

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 965

Galluzzi, L., Vitale, I., Kepp, O., Kroemer, G. (Eds.)

2013, XII, 538 p. 85 illus., 55 illus. in color.

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  • Methods cover cover all biochemical and functional manifestations of senescence at the cellular level
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Content Level » Professional/practitioner

Keywords » autophagy - biochemical manifestations - cell cycle-arresting proteins - cell death - chemokyne signaling - functional manifestations - heterochromatin foci - mammalian cells - model organisms - morphological features - secretory phenotype

Related subjects » Cell Biology

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