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Life Sciences - Cell Biology | Progenitor Cells - Methods and Protocols

Progenitor Cells

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 916

Mace, Kimberly A., Braun, Kristin M. (Eds.)

2012, XIV, 408 p. 67 illus., 50 illus. in color.

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  • Seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems
  • Provides step-by-step detail essentail for reproducible results
  • Contains key notes and implementation advice from the experts

Progenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types.  This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In, Progenitor Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to investigate progenitor cells. These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.



Authoritative and practical, Progenitor Cells : Methods and Protocols seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems.

Content Level » Professional/practitioner

Keywords » cell behavior - cell plasticity - cell types - human progenitor cells - in vitro - microscopy techniques - progenitor cells - regenerative medicine - resident stem - stem cells

Related subjects » Cell Biology - Life Sciences

Table of contents 

Part I.  In vitro Culture Systems

1. Isolation of Satellite Cells from Single Muscle Fibres from Young, Aged or Dystrophic Muscles

Valentina Di Foggia and Lesley Robson

2. Terminal Differentiation of Human Epidermal Stem Cells on Micro-patterned Substrates

John T. Connelly

3. Isolation, Culture and Potentiality Assessment of Lung Alveolar Stem Cells

Feride Oeztuerk-Winder and Juan-Jose Ventura

4. Three-dimensional In Vitro Culture Techniques for Mesenchymal Stem Cells

Fatima A. Saleh, Jessica E. Frith, Jennifer A. Lee, Paul G. Genever

5. Isolation of Adult Stem cells and their Differentiation to Schwann Cells

Cristina Mantovani, Giorgio Terenghi and Susan G Shawcross

6. Functional Purification of Human and Mouse Mammary Stem Cells

Daniela Tosoni, Pier Paolo Di Fiore, Salvatore Pece

7. Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells

S.Bahram Bahrami, Mandana Veiseh, Nancy J.Boudreau

Part II.  Non-mammalian Model Systems

8. Transcriptome Analysis of Drosophila Neural Stem Cells

Katrina S. Gold and Andrea H. Brand

9. Live Imaging for Studying Asymmetric Cell Division in the C. elegans Embryo

Alexia Rabilotta, Rana Amini, and Jean-Claude Labbé

10. Tol2-mediated Gene Transfer and in ovo Electroporation of the Otic Placode:  A Powerful and Versatile Approach for Investigating Embryonic Development and Regeneration of the Chicken Inner Ear

Stephen Freeman, Elena Chrysostomou, Koichi Kawakami, Yoshiko Takahashi, and Nicolas Daudet

11. Labelling Primitive Myeloid Progenitor Cells in Xenopus

Ricardo Costa, Yaoyao Chen,Roberto Paredes, and Enrique Amaya

12. Identification of Oocyte Progenitor Cells in the Zebrafish Ovary

Bruce W. Draper

13. FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms

Belen Tejada Romero, Deborah J Evans and A. Aziz Aboobaker

14. Clonal and Lineage Analysis of Melanocyte Stem Cells (MSCs) and their Progeny in the Zebrafish

Robert C. Tryon and Stephen L. Johnson

15. Reconstitution of the Central Nervous System During Salamander Tail Regeneration from the Implanted Neurospheres

Levan Mchedlishvili, Vladimir Mazurov, Elly M. Tanaka

16. Following the Fate of Neural Progenitors by Homotopic/homochronic Grafts in Xenopus Embryos

Raphael Thuret, Nancy Papalopulu                                                                                    

Section III.  Mammalian Model Systems

17. Analysing the Angiogenic Potential of Gr-1+CD11b+ immature Myeloid Cells from Murine Wounds

Elahe Mahdipour and Kimberly A. Mace

18. In vivo Imaging of Hematopoietic Stem Cells in the Bone Marrow Niche

Oliver Barrett, Roberta Sottocornola and Cristina Lo Celso

19. Characterising the Phenotype of Murine Epidermal Progenitor Cells: Complementary Wholemount Visualisation and Flow Cytometry Strategies

Korinna D. Henseleit, Ann P. Wheeler, Gary Warnes, and Kristin M. Braun

20. Murine Aggregation Chimeras and Wholemount Imaging in Airway Stem Cell Biology

Ian R. Rosewell and Adam Giangreco

21. Live Imaging of Primitive Endoderm Precursors in the Mouse Blastocyst

Joanna B. Grabarek and Berenika Plusa

Section IV. Human Systems

 22. Utilising DNA Mutations to Trace Epithelial Cell Lineages in Human Tissues

Sebastian Zeki, Trevor A Graham & Stuart A C McDonald

23. NF-Ya Protein Delivery As a Tool for Hematopoietic Progenitor Cell Expansion

Alevtina D Domashenko, Susan Weiner, Stephen G Emerson


24. Exploring the Link Between Human Embryonic Stem Cell Organization and Fate using Tension-calibrated Extracellular Matrix Functionalized Polyacrylamide Gels

Johnathon N. Lakins, Andrew R. Chin, and Valerie M. Weaver

25. Isolation of Circulating Angiogenic Cells

Erin E. Vaughan and Timothy O’Brien

26. Methods for Characterisation / Manipulation of Human Corneal Stem Cells and their Applications in Regenerative Medicine

Francesca Corradini, Beatrice Venturi , Graziella Pellegrini and Michele De Luca

27. ALDH as a Marker for Enriching Tumorigenic Human Colonic Stem Cells

Anitha Shenoy, Elizabeth Butterworth, Emina H. Huang

28. Protocols for Investigating microRNA Functions in Human Neural Progenitor Cells

Sandra Almeida, Celine Delaloy, Lei Liu, Fen-Biao Gao

Francesca Corradini, Beatrice Venturi , Graziella Pellegrini and Michele De Luca

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