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Life Sciences - Cell Biology | Human Embryonic Stem Cells Handbook

Human Embryonic Stem Cells Handbook

Series: Methods in Molecular Biology, Vol. 873

Turksen, Kursad (Ed.)

2012, XI, 313 p. 53 illus., 17 illus. in color.

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  • Covers isolation characterization of numerous human embryonic stem cells as well as essential protocols in HES characterization
  • Features detailed, cutting-edge methods and protocols
  • Includes expert tips and key implementation advice for successful implementation

Considerable advances have taken place since the initial isolation and characterization of human embryonic stem (HES) cells; however, significant challenges remain before their potential for restoration and regeneration processes in patients can be realized. Understanding the diversity amongst HES cell lines and realizing the ability to isolate lines with robust differentiation potential remain difficult.  In the Human Embryonic Stem Cells Handbook, experts in the field provide an assortment of protocols that have been used by various laboratories around the world so as to allow both novices and experienced investigators to compare and contrast different approaches to HES cell isolation and characterization with the hope that, from these protocols, researchers might standardize approaches for HES cell biology.  Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Human Embryonic Stem Cells Handbook serves as a valuable reference for scientists pursuing this vital field and its enormous potential.

Content Level » Professional/practitioner

Keywords » HESC - Human embryonic stem cells - Regenerative medicine - Restorative properties - Stem cell characterization - Stem cell isolation

Related subjects » Cell Biology - Life Sciences

Table of contents 

1. Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology

            Ana Maria Fraga, Érica Sara Souza de Araújo, Raquel Stabellini, Naja Vergani, and Lygia V. Pereira

 

2. Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview

            Udaykumar Kolkundkar, Mohan Vemuri, Geetha.M.Swamilingiah, Shruthi Pal, and Jasmeet Kaur

 

3. Human Embryonic Stem Cells Derived in Xeno-Free Conditions

            Amparo Galán Albiñana and Carlos Simón Vallés

 

4. Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark

            Linda Harkness and Moustapha Kassem

 

5. Principles for Derivation of Human Embryonic Stem Cells

            Mikael C.O. Englund, Catharina Ellerström, Katarina Andersson, Karin Noaksson, and Johan Hyllner

 

6. Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells

            Guoliang Meng and Derrick E. Rancourt

 

7. Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos

            Ileana Mateizel, Mieke Geens, Hilde Van de Velde, and Karen Sermon

 

8. Analysis of LINE-1 Expression in Human Pluripotent Cells

            Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia, Santiago Morell, and Jose L. Garcia-Perez

 

9. Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan

            Steven Shoei-Lung Li

 

10. Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos

            Ronak Shetty and Maneesha S. Inamdar

 

11. Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos

            Juan-Carlos Biancotti and Neta Lavon

 

12. Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders

            Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, and Dalit Ben-Yosef

 

13. Single-Cell Enzymatic Dissociation of hESC Lines OxF1--OxF4 and Culture in Feeder-Free Conditions

            Frances A. Brook

 

14. Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension

            Hossein Baharvand,  Mehran Rezaei Larijani, and Maryam Yousefi

 

15. Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells

            Roman Krawetz and Derrick E. Rancourt

 

16. Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs)

            Khun-Hong Lie, Henry Chung, and Kuldip S. Sidhu

 

17. Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol

            Dezhong Yin, Tahereh Tavakoli, Wei-Qiang Gao, and Wu Ma

 

18. Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells

            Aaron M. Elliott, Kristi A. Hohenstein Elliott, and Anja Kammesheidt

 

19. Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells

            Marc Jung and James Adjaye

 

20. Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq)

            Christina Grimm and James Adjaye

 

21. Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic Analysis

            Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem, and Blagoy Blagoev

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