Editors:
- Features techniques specific to both male and female germlines
- Provides easily accessible, step-by-step protocols that are ready for the lab
- Includes expert tips and implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 825)
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Table of contents (18 protocols)
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Front Matter
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Male Germline
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Front Matter
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Female Germline
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Front Matter
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About this book
The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both the male and female germline. As the techniques used for isolating and studying male and female germ cells are slightly dissimilar, the different technologies are grouped under two sections in this volume, namely, male germline and female germline. This collection brings the field of germline developmental studies together such that readers can find, in a single volume, techniques that allow the isolation of gonads with germ cells at different stages of development, the study of the genomics and epigenomics of germ cells, and an examination of the role of single genes in the developmental potential and function of germ cells. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and the avoidance of known pitfalls.Â
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Comprehensive and cutting-edge, Germline Development: Methods and Protocols serves as a comprehensive text to individuals, particularly researchers in reproductive and developmental biology, who are interested in studying cells of the germ lineage.
Editors and Affiliations
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, School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong/PR China
Wai-Yee Chan
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, Animal Biosciences, ARS-USDA, Beltsville, USA
Le Ann Blomberg
Bibliographic Information
Book Title: Germline Development
Book Subtitle: Methods and Protocols
Editors: Wai-Yee Chan, Le Ann Blomberg
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-436-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-435-3Published: 06 December 2011
Softcover ISBN: 978-1-4939-5883-2Published: 23 August 2016
eBook ISBN: 978-1-61779-436-0Published: 07 December 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 256
Topics: Cell Biology, Developmental Biology