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Human Embryonic and Induced Pluripotent Stem Cells

Lineage-Specific Differentiation Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Kaiming Ye

    1. , College of Engineering, University of Arkansas, Fayetteville, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Sha Jin

    1. , College of Engineering, University of Arkansas, Fayetteville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Serves to inspire the next generation of regenerative medicine and tissue engineering technologies
  • Features an extensive collection of protocols developed by leading scientists in the field
  • Contains practical laboratory guidance to promote successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Maintenance and Cryopreservation of ES and iPS Cells

    1. Front Matter

      Pages 1-1
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  3. Maintenance and Cryopreservation of ES and iPS cells

    1. Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

      • Dong-Youn Hwang
      Pages 3-12

    2. Growth of Human Embryonic Stem Cells in Long-Term Hypoxia

      • Vladimir Zachar, Simon C. Weli, Mayuri S. Prasad, Trine Fink
      Pages 13-24

    3. Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells

      • Masato Nagaoka, Stephen A. Duncan
      Pages 25-35

    4. Scale-Up of Single Cell–Inoculated Suspension Cultures of Human Embryonic Stem Cells

      • Harmeet Singh, Pamela Mok, Robert Zweigerdt
      Pages 37-50

    5. Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells

      • Guang-wei Sun, Xiao-xi Xu, Nan Li, Ying Zhang, Xiao-jun Ma
      Pages 51-58

    6. Expansion of Pluripotent Stem Cells in Defined, Xeno-Free Culture System

      • Kristiina Rajala
      Pages 59-69

    7. Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells

      • Behrouz Aflatoonian, Harry D. Moore
      Pages 71-83

    8. Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

      • Frida Holm
      Pages 85-90

  4. Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming

    1. Front Matter

      Pages 91-91
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    2. Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2

      • Alessandra Giorgetti, Nuria Montserrat, Juan Carlos Izpisua Belmonte
      Pages 93-111

    3. Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

      • Naoki Nishishita, Chiemi Takenaka, Shin Kawamata
      Pages 113-131

    4. Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage

      • Maria V. Shutova, Ilya V. Chestkov, Alexandra N. Bogomazova, Maria A. Lagarkova, Sergey L. Kiselev
      Pages 133-149

    5. Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells

      • Hua Liu, Su Mi Choi, Yoon-Young Jang
      Pages 151-160

    6. Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

      • Kazutoshi Takahashi
      Pages 161-171

    7. Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)

      • Hiroe Ohnishi, Yasuaki Oda, Hajime Ohgushi
      Pages 173-190

    8. Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes

      • Athanasia D. Panopoulos, Sergio Ruiz, Juan Carlos Izpisua Belmonte
      Pages 191-201

    9. Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles

      • Ann Peters, Elias T. Zambidis
      Pages 203-227

  5. Generation of Patient-Specific iPS Cells for Clinical Application

    1. Front Matter

      Pages 229-229
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Keywords

About this book

Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world.  The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies.  Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls.

 

Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.

Editors and Affiliations

  • , College of Engineering, University of Arkansas, Fayetteville, USA

    Kaiming Ye, Sha Jin

Bibliographic Information

  • Book Title: Human Embryonic and Induced Pluripotent Stem Cells

  • Book Subtitle: Lineage-Specific Differentiation Protocols

  • Editors: Kaiming Ye, Sha Jin

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-61779-267-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-266-3Published: 07 September 2011

  • Softcover ISBN: 978-1-4939-5893-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-267-0Published: 08 September 2011

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XVI, 486

  • Number of Illustrations: 40 b/w illustrations, 48 illustrations in colour

  • Topics: Cell Biology, Stem Cells

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