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Life Sciences - Cell Biology | Permeability Barrier - Methods and Protocols

Permeability Barrier

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 763

Turksen, Kursad (Ed.)

2011, XV, 450p. 101 illus., 22 illus. in color. With online files/update.

A product of Humana Press
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  • Includes cutting-edge methods and protocols covering a variety of permeability barrier
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and vital implementation advice from the experts

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body.  In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Content Level » Professional/practitioner

Keywords » Blood retina barrier - Blood-brain barrier - Epidermal barrier formation - Epithelial cells - In vitro - In vivo - Membranes

Related subjects » Cell Biology

Table of contents 

1. Computational Modeling of the Skin Barrier

            Arne Naegel, Michael Heisig, and Gabriel Wittum


2. In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles

            Ana Melero, Tsambika Hahn, Ulrich F. Schaefer, and Marc Schneider


3. Transcriptional Regulation of Epidermal Barrier Formation

            Ambica Bhandari, Michael L. Salmans, William Gordon, and Bogi Andersen


4. Epidermal Permeability Barrier  (EPB) Measurement in Mammalian Skin

            Arup Kumar Indra and Mark Leid


5. Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation

            Brent M. Bany and G. Scot Hamilton


6. Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests

            Willemijn E. Corpeleijn, Ruurd M. van Elburg, Ido P. Kema, and Johannes B. van Goudoever


7. Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium

            Carrie A. Duckworth and Alastair J. Watson


8. Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells

            Rino P Donato, Adaweyah El-Merhibi, Batjargal Gundsambuu, Kai Yan Mak, Emma R Formosa, Xian Wang, Catherine A Abbott, and Barry C Powell


9. Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux

            Barry Press


10. Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

            Isabel Larre, Ruben G. Contreras, and Marcelino Cereijido


11. Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging

            Judith Lechner, Daniel Hekl, Helmut Gatt, Markus Voelp, and Thomas Seppi


12. Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy

            Marta Garcia-Ramírez, Marta Villarroel, Lídia Corraliza, Cristina Hernández, and Rafael Simó


13. Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells

            Monika Strengert and Ulla G. Knaus


14. Permeability of Differentiated Human Urothelium In Vitro

            Peter Rubenwolf and Jennifer Southgate


15. Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry

            Séverine Mazaud-Guittot, Alexander Gow, and Brigitte Le Magueresse-Battistoni


16. An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics

            Dolores D. Mruk and C. Yan Cheng


17. Analysis of Endothelial Barrier Function In Vitro

            Yuping Wang and J. Steven Alexander


18. Role of Endothelial Cell-Cell Junctions in Endothelial Permeability

            Armelle Le Guelte and Julie Gavard


19. In Vitro Analyses of Endothelial Cell Permeability

            Elizabeth Monaghan-Benson and Erika S. Wittchen


20. Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells

            Kristina Giantsos, Mark Cluff, and Randal Dull


21. Role of Caveolin-1 in the Regulation of Pulmonary Endothelial Permeability

            Yu Sun, Richard D. Minshall, and Guochang Hu


22. Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers

            Andreas Ludwig, Anselm Sommer, and Stefan Uhlig


23. Permeability of Endothelial Barrier: Cell Culture and In Vivo Models

            Alexander N. Garcia, Stephen M. Vogel, Yulia A. Komarova, and Asrar B. Malik


24. Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability

            Matthew Campbell and Peter Humphries


25. Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase

            Mehmet Kaya and Bulent Ahishali


26. In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier

            Nadia Caram-Salas, Eve Boileau, Graham Farrington, Ellen Garber, Eric Brunette, Abedelnasser Abulrob, and Danica Stanimirovic


27. Evaluation of VEGF-Induced Vascular Permeability in Mice

            Sara M. Weis


28. In Vivo Measurement of Glioma-Induced Vascular Permeability

            Jisook Lee, Andrew Baird, and Brian P. Eliceiri


29. In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption

            Abedelnasser Abulrob, Eric Brunette, Jacqueline Slinn, Ewa Baumann, and Danica Stanimirovic

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