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Life Sciences - Cell Biology | Claudins - Methods and Protocols


Methods and Protocols

Series: Methods in Molecular Biology, Vol. 762

Turksen, Kursad (Ed.)

2011, XIV, 447p. 88 illus., 33 illus. in color.

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  • Collects key practical protocols for the study of claudins
  • Serves as the first comprehensive volume of techniques involving this subject
  • Features tips and vital implementation advice to ensure success

Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles.  In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Content Level » Professional/practitioner

Keywords » Cell polarity - Permeability - Tight junctions - Tissue specific roles - Trafficking

Related subjects » Cell Biology

Table of contents 

1. Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling

            Christina M. Van Itallie and James M. Anderson


2. Biochemical Analysis of Claudin Binding Compatibility

            Christina Ward and Michael Koval


3. Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines

            Alan S.L. Yu


4. The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Status

            Makoto Osanai


5. Interactions between Clostridium perfringens Enterotoxin and Claudins

            Susan L. Robertson and Bruce A. McClane


6. Biophysical Methods to Probe Claudin Mediated Adhesion at the Cellular and Molecular Level

            Sri Ram Krishna Vedula, Tong Seng Lim, Walter Hunziker, and Chwee Teck Lim


7. Detection of Tight Junction Barrier Function In Vivo by Biotin

            Lei Ding, Yuguo Zhang, Rodney Tatum, and Yan-Hua Chen


8. The Co-Culture Method to Examine Interactions between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells

            Tetsuichiro Inai


9. Claudin 4: Functional Studies Beyond the Tight Junction

            Holly A. Eckelhoefer, Thejani E. Rajapaksa, Jing Wang, Mary Stover-Hamer, Nancy C. Appleby, Jun Ling, and David D. Lo


10. Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin 16

            P. Jaya Kausalya and Walter Hunziker


11. Claudin Family Proteins in C. elegans

            Jeffrey S.Simske and Jeff Hardin


12. In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka

            Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki


13. Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium

            Phuong Bui and Scott P. Kelly


14. Manipulating Claudin Expression in Avian Embryos

            Michelle M. Collins and Aimee K. Ryan


15. Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues

            Lorenza González-Mariscal, Erika Garay, and Miguel Quirós


16. Expression and Function of Claudins in Hepatocytes

            Takashi Kojima and Norimasa Sawada


17. Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture

            Junko Fujita-Yoshigaki


18. Development of Biological Tools to Study Claudins in the Male Reproductive Tract

            Daniel G. Cyr, Évemie Dubé, Julie Dufresne, and Mary Gregory


19. Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo

            Julian A. Guttman


20. Laboratory Methods in the Study of Endometrial Claudin-4

            Paulo Serafini, André Monteiro Rocha, Gary Daniel Smith, Eduardo Leme Alves da Motta, and Edmund Chada Baracat


21. Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction

            Yu Sun, Richard D. Minshall, and Guochang Hu


22. Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells

            Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic


23. Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier

            Heping Xu and Janet Liversidge


24. MMP-Mediated Disruption of Claudin-5 in the Blood-Brain Barrier of Rat Brain after Cerebral Ischemia

            Yi Yang and Gary A. Rosenberg


25. Claudin-5 Expression in In Vitro Models of the Blood-Brain Barrier

            Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I.  Teichberg


26. HIV-1-Induced Alterations of Claudin-5 Expression at the Blood Brain Barrier Level

            Ibolya E. András and Michal Toborek


27. Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Biopsies

            Jane E. Collins, Adam Kirk, Sara K. Campbell, Juan Mason, and Susan J. Wilson


28. Claudin-16/Paracellin-1, Cloning, Expression, and Its Role in Tight Junction Functions in Cancer and Endothelial Cells

            Tracey A. Martin and Wen G. Jiang


29. Dynamics of Claudins Expression in Colitis and Colitis-Associated Cancer in Rat

            Yoshiaki Arimura, Kanna Nagaishi, and Masayo Hosokawa


30. Anti-Claudin 4 Conjugated Highly Luminescence Nanoparticles as Biological Labels for Pancreatic Cancer Sensing

            Ken-Tye Yong

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