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  • © 2011

Mammalian Cell Viability

Methods and Protocols

  • Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment
  • Facilitates the multiplexing of methods for the greatest levels of success
  • Contains expert tips and key implementation advice

Part of the book series: Methods in Molecular Biology (MIMB, volume 740)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Cell Viability Assays: Introduction

    • Martin J. Stoddart
    Pages 1-6
  3. Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

    • Kristine S. Louis, Andre C. Siegel
    Pages 7-12
  4. The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability

    • Ning Ke, Xiaobo Wang, Xiao Xu, Yama A. Abassi
    Pages 33-43
  5. Annexin V/7-AAD Staining in Keratinocytes

    • Maya Zimmermann, Norbert Meyer
    Pages 57-63
  6. Measurement of Caspase Activity: From Cell Populations to Individual Cells

    • Gabriela Paroni, Claudio Brancolini
    Pages 65-79
  7. Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes

    • Donald Wlodkowic, Joanna Skommer, Zbigniew Darzynkiewicz
    Pages 81-89
  8. Multiplexing Cell Viability Assays

    • Helga H. J. Gerets, Stéphane Dhalluin, Franck A. Atienzar
    Pages 91-101
  9. Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death

    • Terry L. Riss, Richard A. Moravec, Andrew L. Niles
    Pages 103-114
  10. Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers

    • Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, Sibylle Grad
    Pages 127-140
  11. Exploiting the Liberation of Zn2+ to Measure Cell Viability

    • Christian J. Stork, Yang V. Li
    Pages 157-163
  12. Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs

    • Karim Oudina, Adeline Cambon-Binder, Delphine Logeart-Avramoglou
    Pages 165-178
  13. Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test

    • Sophie Verrier, Alina Zoladek, Ioan Notingher
    Pages 179-189

About this book

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Editors and Affiliations

  • AO Research Institute, Davos Platz, Switzerland

    Martin J. Stoddart

Bibliographic Information

  • Book Title: Mammalian Cell Viability

  • Book Subtitle: Methods and Protocols

  • Editors: Martin J. Stoddart

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-108-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-107-9Published: 12 April 2011

  • Softcover ISBN: 978-1-4939-6153-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-108-6Published: 06 April 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 240

  • Topics: Cell Biology, Cell Culture

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access