3D Cell Culture

1. Front Matter

   Pages i-xi

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2. 3D Cell Culture: A Review of Current Approaches and Techniques

   • John W. Haycock

   Pages 1-15

3. Scaffolds for Tissue Engineering and 3D Cell Culture

   • Eleonora Carletti, Antonella Motta, Claudio Migliaresi

   Pages 17-39

4. Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning Microscopy

   • Vanessa Hearnden, Sheila MacNeil, Giuseppe Battaglia

   Pages 41-51

5. Using Immuno-Scanning Electron Microscopy for the Observation of Focal Adhesion-substratum interactions at the Nano- and Microscale in S-Phase Cells

   • Manus J. P. Biggs, R. Geoff Richards, Matthew J. Dalby

   Pages 53-60

6. 3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors

   • Sarah H. Cartmell, Sarah Rathbone, Gemma Jones, L. Araida Hidalgo-Bastida

   Pages 61-76

7. Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs

   • David A. Lee, June Brand, Donald Salter, Oto-Ola Akanji, Tina T. Chowdhury

   Pages 77-97

8. Genetic Modification of Chondrocytes Using Viral Vectors

   • Teresa Coughlan, Aileen Crawford, Paul Hatton, Michael Barker

   Pages 99-114

9. Stem Cell and Neuron Co-cultures for the Study of Nerve Regeneration

   • Paul J. Kingham, Cristina Mantovani, Giorgio Terenghi

   Pages 115-127

10. Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use

    • Sheila MacNeil, Joanna Shepherd, Louise Smith

    Pages 129-153

11. Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair

    • Celia Murray-Dunning, Sally L. McArthur, Tao Sun, Rob McKean, Anthony J. Ryan, John W. Haycock

    Pages 155-166

12. Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization

    • Rui C. Pereira, Chiara Gentili, Ranieri Cancedda, Helena S. Azevedo, Rui L. Reis

    Pages 167-181

13. Micro-structured Materials and Mechanical Cues in 3D Collagen Gels

    • James B. Phillips, Robert Brown

    Pages 183-196

14. Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract

    • Claire L. Varley, Jennifer Southgate

    Pages 197-211

15. Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial–Neuroectodermal–Mesenchymal Interaction System

    • Desmond J. Tobin

    Pages 213-227

16. Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials

    • Ronald E. Unger, Sven Halstenberg, Anne Sartoris, C. James Kirkpatrick

    Pages 229-241

17. Assessment of Nanomaterials Cytotoxicity and Internalization

    • Noha M. Zaki, Nicola Tirelli

    Pages 243-259

18. Practical Aspects of OCT Imaging in Tissue Engineering

    • Stephen J. Matcher

    Pages 261-280

19. Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture

    • Lee Buttery, Robert Bielby, Daniel Howard, Kevin Shakesheff

    Pages 281-308

20. 3D Structuring of Biocompatible and Biodegradable Polymers Via Stereolithography

    • Andrew A. Gill, Frederik Claeyssens

    Pages 309-321

Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.

• Bioreactor design
• Growth environment
• Regenerative medicine
• Scaffolds
• Stem cell technology
• Three dimensional models
• Tissue engineering
• Cell culture

From the reviews:

“This book is an excellent resource to help researchers find practical information to enable them to develop three-dimensional cell culture systems. … anyone who wishes to enter this field should be able to use this book to develop their own bespoke 3D system. In conclusion, I would recommend this book as being very useful to a wide audience … . will be essential for graduate students and postdocs as well as to investigators wishing to expand their work into 3D cell culture and tissue engineering.” (Julian B. Chaudhuri, ChemBioChem, Vol. 12, 2011)

“A necessary book for all who use cell culture. No other words can better tell the reader about this compendium of the most innovative culture techniques we hold to try to reach the ultimate goal of cell culture … . For those who wish to enter the field, some introductory reviews explain the present-day challenges in 3D culture while more detailed protocols talk about bioreactors and agarose constructs.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

• Kroto Research Institute, Department of Engineering Materials, University of Sheffield, Sheffield, United Kingdom

  John W. Haycock

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