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Life Sciences - Cell Biology | Transcription Factors - Methods and Protocols

Transcription Factors

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 647

Higgins, Paul J. (Ed.)

2010, XIV, 377 p.

A product of Humana Press
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  • The ‘methods’ chapters are preceded by several excellent reviews that highlight the role of post-translational modifications and subcellular trafficking in transcription factor
  • Includes new techniques for assessing kinase targeting of specific transcription factors, sites of phosphorylation and the involved signaling pathways
  • Describes novel molecular and elegant microscopic imaging-based procedures to evaluate nuclear translocation and export in living cells

In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today. 

Content Level » Professional/practitioner

Keywords » DNA - Intracellular trafficking - Kinase targeting - Nuclear translocation - Phosphorylation - Post-translational modifications - Protein degradation - Regulation - Termination - Translation - gene expression - genes - molecular control - molecular mechanisms - post-translational modification

Related subjects » Cell Biology

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