Retinoids

1. Front Matter

   Pages i-x

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2. Quantification of Endogenous Retinoids

   • Maureen A. Kane, Joseph L. Napoli

   Pages 1-54

3. Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids

   • Jane Hu, Dean Bok

   Pages 55-73

4. Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol

   • Jaspal S. Khillan, Liguo Chen

   Pages 75-83

5. In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists

   • Masahiro Kono, Rosalie K. Crouch

   Pages 85-94

6. Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments

   • Vladimir J. Kefalov, M. Carter Cornwall, Gordon L. Fain

   Pages 95-114

7. Measurement of the Mobility of All-Trans-Retinol with Two-Photon Fluorescence Recovery After Photobleaching

   • Yiannis Koutalos

   Pages 115-127

8. Microfluorometric Measurement of the Formation of All-Trans-Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors

   • Yiannis Koutalos, M. Carter Cornwall

   Pages 129-147

9. HPLC / MSN Analysis of Retinoids

   • James E. Evans, Peter McCaffery

   Pages 149-162

10. Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration

    • Ming Zhong, Robert S. Molday

    Pages 163-176

11. Fluorescence-Based Technique for Analyzing Retinoic Acid

    • Leslie J. Donato, Noa Noy

    Pages 177-187

12. The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy

    • Claudia Folli, Roberto Favilla, Rodolfo Berni

    Pages 189-207

13. Assay of Retinol-Binding Protein–Transthyretin Interaction and Techniques to Identify Competing Ligands

    • Nathan L. Mata, Kim Phan, Yun Han

    Pages 209-227

14. Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle

    • Marcin Golczak, Grzegorz Bereta, Akiko Maeda, Krzysztof Palczewski

    Pages 229-245

15. Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy

    • Yoshikazu Imanishi, Krzysztof Palczewski

    Pages 247-261

16. Reverse-Phase High-Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues

    • Youn-Kyung Kim, Loredana Quadro

    Pages 263-275

17. Detection of Retinoic Acid Catabolism with Reporter Systems and by In Situ Hybridization for CYP26 Enzymes

    • Yasuo Sakai, Ursula C. Dräger

    Pages 277-294

18. Diet in Vitamin A Research

    • A. Catharine Ross

    Pages 295-313

19. Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium

    • Janet R. Sparrow, So Ra Kim, Yalin Wu

    Pages 315-327

20. Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina

    • Gabriel H. Travis, Joanna Kaylor, Quan Yuan

    Pages 329-339

New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A’s impact on the human body.

• Transport
• Vitamin
• Vivo
• biological functions
• cells
• enzymes
• hybridization
• molecular biology
• tissue

From the reviews:

“This excellent book provides authoritative and cutting-edge information in the field of retinoids … . It is intended both for beginning and experienced researchers in diverse fields who are interested in learning new techniques. Even graduate students, nutritionists, and clinicians could find this book informative and helpful. … By combining biochemical, biophysical, and cell biological techniques, the book thoroughly addresses the field of retinoid research.” (Omer Iqbal, Doody’s Review Service, October, 2010)

• David Geffen School of Medicine, Dept. Physiology, University of California, Los Angeles, Los Angeles, USA

  Hui Sun

• , Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, USA

  Gabriel H. Travis

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