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Life Sciences - Cell Biology | Mouse Molecular Embryology - Methods and Protocols

Mouse Molecular Embryology

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1092

Volume package: Mouse Molecular Embryology

Lewandoski, Mark (Ed.)

2014, XIV, 465 p. 107 illus., 71 illus. in color.

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  • Presents cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study  mouse embryology.  These include protocols and techniques that  are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA.  With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

  

Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

Content Level » Professional/practitioner

Keywords » conditional gene inactivation - culturing explanted embryonic tissue - fluorescence imaging - genetic ablation - genetic technologies - harvesting embryonic RNA - human disease - lineage tracing - manipulating embryonic gene expression - mouse embryo

Related subjects » Cell Biology - Evolutionary & Developmental Biology

Table of contents 

1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining

Yoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina

 

2. Two Color In Situ Hybridization of Whole-Mount Mouse Embryos

Kristin K. Biris and Terry P. Yamaguchi

 

3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections

Davide De Pietri Tonelli, Yoanne M. Clovis and  Wieland B. Huttner

 

4. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays

Latasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar and Joyce A. Lloyd

 

5. EMAGE: Electronic Mouse Atlas of Gene Expression

Lorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman. Yiya Yang, Nick Burton, Jianguo Rao, Jeffrey H. Christiansen, Richard A. Baldock and Duncan R. Davidson

 

6. Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue

Eric Villalon, David J. Schulz and Samuel T. Waters

 

7. Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach

Aimin Liu and Jonathan Eggenschwiler

 

8. Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression

David A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert and Patrick P. L. Tam

 

9. Generation of Tissue Organoids by Compaction Reaggregation

Julie M. Sheridan and C. Clare Blackburn

 

10. Ultra-Rapid Vitrification of Mouse Oocytes and Embryos

Mark G. Larman PhD and David K. Gardner

 

11. Mammalian Preimplantation Embryo Culture

David K. Gardner and Michelle Lane

 

12. Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs

Julie Gordon, Billie A. Moore, C. Clare Blackburn and

Nancy R. Manley

 

13. Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia

Yvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran, Junichi Nakai and Michael I. Kotlikoff

 

14. Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms

Suresh I. Prajapati, Lisa Nevell  and Charles Keller

 

15. Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos

Daisuke Sakai and Paul A Trainor

 

16. Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues

Kaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi, Prabhakar R. Gudla and Stephen J. Lockett

 

17.     Protein/Peptide Transduction in Metanephric Explant Culture

Sergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, and Alan O. Perantoni

 

18. Direction of Cells Programmed to Die in Mouse Embroys

Rocío Hernández-Martínez and Luis Covarrubias

 

19. Microscopic Computed Tomography-based  Virtual Histology of Embryos

Suresh I. Prajapati, David R. Rodriguez, Charles Keller

 

20. Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study

Yelena Golubeva and David Symer

 

21. The Latest Improvements in the Mouse Sperm Preservation

Takehito Kaneko

 

22. Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro

Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam

 

23. Using the Textpresso Site Specific Recombinases Web Server to Identify  Cre Expressing Mouse Strains and Floxed Alleles

Brian G. Condie and William M. Urbanski

 

24.  Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing

Sonja Nowotschin and Anna-Katerina Hadjantonakis

 

25. Genetic Cell Ablation

Damien Grégoire and Marie Kmita

 

26. Essentials of Recombinase-based Genetic Fate Mapping in Mice

Patricia Jensen and Susan M. Dymecki

 

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