Logo - springer
Slogan - springer

Life Sciences - Cell Biology | Mouse Molecular Embryology - Methods and Protocols

Mouse Molecular Embryology

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1092

Volume package: Mouse Molecular Embryology

Lewandoski, Mark (Ed.)

2014, XIV, 465 p. 107 illus., 71 illus. in color.

Available Formats:

Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.

You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.

After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.


(net) price for USA

ISBN 978-1-60327-292-6

digitally watermarked, no DRM

Included Format: PDF and EPUB

download immediately after purchase

learn more about Springer eBooks

add to marked items


Hardcover version

You can pay for Springer Books with Visa, Mastercard, American Express or Paypal.

Standard shipping is free of charge for individual customers.


(net) price for USA

ISBN 978-1-60327-290-2

free shipping for individuals worldwide

usually dispatched within 3 to 5 business days

add to marked items

  • Presents cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

In Mouse Molecular Embryology: Methods and Protocols, expert researchers in the field detail many of the protocols used to study  mouse embryology.  These include protocols and techniques that  are "close to the embryo": such as, manipulating embryonic gene expression, culturing explanted embryonic tissue and harvesting embryonic RNA.  With additional chapters on fluorescence imaging, lineage tracing, and genetic ablation. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Mouse Molecular Embryology: Methods and Protocols seeks to aid scientist in the further study of mouse embryo and its relation to other aspects of biological research.

Content Level » Professional/practitioner

Keywords » conditional gene inactivation - culturing explanted embryonic tissue - fluorescence imaging - genetic ablation - genetic technologies - harvesting embryonic RNA - human disease - lineage tracing - manipulating embryonic gene expression - mouse embryo

Related subjects » Cell Biology - Evolutionary & Developmental Biology

Table of contents 

1. In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional â-galactosidase Staining

Yoshihiro Komatsu, Satoshi Kishigami, and Yuji Mishina


2. Two Color In Situ Hybridization of Whole-Mount Mouse Embryos

Kristin K. Biris and Terry P. Yamaguchi


3. Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections

Davide De Pietri Tonelli, Yoanne M. Clovis and  Wieland B. Huttner


4. Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays

Latasha C. Redmond, Christopher J. Pang, Catherine Dumur, Jack L. Haar and Joyce A. Lloyd


5. EMAGE: Electronic Mouse Atlas of Gene Expression

Lorna Richardson, Peter Stevenson, Shanmugasundaram Venkataraman. Yiya Yang, Nick Burton, Jianguo Rao, Jeffrey H. Christiansen, Richard A. Baldock and Duncan R. Davidson


6. Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue

Eric Villalon, David J. Schulz and Samuel T. Waters


7. Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach

Aimin Liu and Jonathan Eggenschwiler


8. Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression

David A. F. Loebel, Tania Radziewic, Melinda Power, Joshua B. Studdert and Patrick P. L. Tam


9. Generation of Tissue Organoids by Compaction Reaggregation

Julie M. Sheridan and C. Clare Blackburn


10. Ultra-Rapid Vitrification of Mouse Oocytes and Embryos

Mark G. Larman PhD and David K. Gardner


11. Mammalian Preimplantation Embryo Culture

David K. Gardner and Michelle Lane


12. Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs

Julie Gordon, Billie A. Moore, C. Clare Blackburn and

Nancy R. Manley


13. Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia

Yvonne Norine Tallini, Kai Su Greene, Bo Shui, Calum William Russell, Jane Constance Lee, Robert Michael Doran, Junichi Nakai and Michael I. Kotlikoff


14. Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms

Suresh I. Prajapati, Lisa Nevell  and Charles Keller


15. Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos

Daisuke Sakai and Paul A Trainor


16. Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues

Kaustav Nandy, Jusub Kim, Dean P. McCullough, Matthew McAuliffe, Karen J. Meaburn, Terry P. Yamaguchi, Prabhakar R. Gudla and Stephen J. Lockett


17.     Protein/Peptide Transduction in Metanephric Explant Culture

Sergey Plisov, Honghe Wang, Nadya Tarasova, Nirmala Sharma, and Alan O. Perantoni


18. Direction of Cells Programmed to Die in Mouse Embroys

Rocío Hernández-Martínez and Luis Covarrubias


19. Microscopic Computed Tomography-based  Virtual Histology of Embryos

Suresh I. Prajapati, David R. Rodriguez, Charles Keller


20. Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study

Yelena Golubeva and David Symer


21. The Latest Improvements in the Mouse Sperm Preservation

Takehito Kaneko


22. Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro

Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam


23. Using the Textpresso Site Specific Recombinases Web Server to Identify  Cre Expressing Mouse Strains and Floxed Alleles

Brian G. Condie and William M. Urbanski


24.  Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing

Sonja Nowotschin and Anna-Katerina Hadjantonakis


25. Genetic Cell Ablation

Damien Grégoire and Marie Kmita


26. Essentials of Recombinase-based Genetic Fate Mapping in Mice

Patricia Jensen and Susan M. Dymecki


Popular Content within this publication 



Read this Book on Springerlink

Services for this book

New Book Alert

Get alerted on new Springer publications in the subject area of Cell Biology.