Human Embryonic Stem Cells

1. Front Matter

   Pages i-xviii

   PDF

2. Policy

   1. Front Matter

      Pages 1-1

      PDF

   2. Ethical Issues Associated with Pluripotent Stem Cells

      • Steve Usdin

      Pages 3-25

   3. A Researcher’s Guide to Federally Funded Human Embryonic Stem Cell Research in the United States

      • Gregory J. Downing

      Pages 27-37

   4. Intellectual Property of Human Pluripotent Stem Cells

      • Mark L. Rohrbaugh

      Pages 39-60

3. Types of Pluripotent Cells

   1. Front Matter

      Pages 61-61

      PDF

   2. Embryonal Carcinoma Cells

      • Jonathan S. Draper, Harry Moore, Peter W. Andrews

      Pages 63-87

   3. Human Pluripotent Stem Cells from Bone Marrow

      • Felipe Prosper, Catherine M. Verfaillie

      Pages 89-111

   4. Protocols for the Isolation and Maintenance of Human Embryonic Stem Cells

      • Melissa K. Carpenter, Chunhui Xu, Christine A. Daigh, Jessica E. Antosiewicz, James A. Thomson

      Pages 113-126

   5. Subcloning and Alternative Methods for the Derivation and Culture of Human Embryonic Stem Cells

      • Michal Amit, Hanna Segev, Dorit Manor, Joseph Itskovitz-Eldor

      Pages 127-141

4. Differentiation

   1. Front Matter

      Pages 143-143

      PDF

   2. Differentiation of Neuroepithelia from Human Embryonic Stem Cells

      • Su-Chun Zhang

      Pages 145-159

   3. Pancreatic Differentiation of Pluripotent Stem Cells

      • Nadya Lumelsky

      Pages 161-179

   4. Human Embryonic Stem Cell-Derived Cardiomyocytes

      • Chunhui Xu, Melissa K. Carpenter

      Pages 181-199

   5. Vascular Lineage Differentiation from Human Embryonic Stem Cells

      • Sharon Gerecht-Nir, Joseph Itskovitz-Eldor

      Pages 201-217

   6. Hematopoietic Progenitors Derived from Human Embryonic Stem Cells

      • Dan S. Kaufman

      Pages 219-236

5. Therapeutics

   1. Front Matter

      Pages 237-237

      PDF

   2. Human Embryonic vs Adult Stem Cells for Transplantation Therapies

      • Calvin B. Harley, Mahendra S. Rao

      Pages 239-264

   3. Genetic Manipulation of Human Embryonic Stem Cells

      • Micha Drukker, Nissim Benvenisty

      Pages 265-284

   4. Human Therapeutic Cloning

      • Jose B. Cibelli

      Pages 285-295

A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.

• cell
• cell lines
• cloning
• embryo
• stem cells
• tissue

"This is an excellent compendium about human stem cells..." -Biologist

• National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, USA

  Arlene Y. Chiu

• National Institute on Aging, National Institutes of Health, Baltimore, USA

  Mahendra S. Rao

Policies and ethics