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Life Sciences - Cell Biology | Wnt Signaling - Volume 1: Pathway Methods and Mammalian Models

Wnt Signaling

Volume 1: Pathway Methods and Mammalian Models

Series: Methods in Molecular Biology, Vol. 468

Volume package: Wnt Signalling

Vincan, Elizabeth (Ed.)

2008

A product of Humana Press
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  • Records our progress to date in understanding the intricacies of Wnt signaling
  • Includes assays for and models of both canonical and non-canonical Wnt signaling pathways
  • Contributing authors are either current or past members of the main laboratories that have shaped the Wnt field
  • Includes detailed methods as well as overviews, providing easy to follow protocols as well as a background/current status of the field

Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study.  Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway.  In Volume 1, Pathway Methods and Mammalian Models, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips.

 

Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource. 

Content Level » Research

Keywords » Activation - Calcium - Canonical Wnt/FZD signaling - Degenerative diseases and cancer - Embryogenesis - Non-canonical signaling - Promoter - Protein-protein interaction - Tissue homeostasis - Wnt/FZD function - genes - molecular biology - proteins - tissue - transcription

Related subjects » Cell Biology

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