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Hepatocyte Transplantation

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Anil Dhawan

    1. King’s College Hospital, London, UK

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  2. Robin D. Hughes

    1. School of Medicine, King’s College London, London, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Brings together all the methodology needed to perform human hepatocyte transplantation for the first time in one volume
  • Key researchers cover all aspects of hepatocyte transplantation from bench to bedside
  • Provides a practical basis for evaluating stem cell-derived hepatocytes for the treatment of liver disease in the future

Part of the book series: Methods in Molecular Biology (MIMB, volume 481)

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Table of contents (18 protocols)

  1. Front Matter

    Pages 1-17
    Download chapter PDF

  2. Human Hepatocyte Transplantation Overview

    • Juliana Puppi, Anil Dhawan
    Pages 1-16

  3. Isolation of Human Hepatocytes

    • Ragai R. Mitry
    Pages 17-23

  4. An Optimised Method for Cryopreservation of Human Hepatocytes

    • Claire Terry, Robin D. Hughes
    Pages 25-34

  5. Liver Cell Culture Techniques

    • José V. Castell, María José Gómez-Lechón
    Pages 35-46

  6. In Vitro Assays for Induction of Drug Metabolism

    • Brian G. Lake, Roger J. Price, Amanda M. Giddings, David G. Walters
    Pages 47-58

  7. Hepatocyte Apoptosis

    • Mustapha Najimi, Françoise Smets, Etienne Sokal
    Pages 59-74

  8. Small Animal Models of Hepatocyte Transplantation

    • Jurgen Seppen, Ebtisam El Filali, Ronald Oude Elferink
    Pages 75-82

  9. Hepatocyte Transplantation Techniques: Large Animal Models

    • Anne Weber, Marie-Thérèse Groyer-Picard, Ibrahim Dagher
    Pages 83-96

  10. Cell Transplant Techniques: Engraftment Detection of Cells

    • Robert A. Fisher, Valeria R. Mas
    Pages 97-105

  11. Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation

    • Yao-Ming Wu, Sanjeev Gupta
    Pages 107-116

  12. Ex Vivo Gene Transfer into Hepatocytes

    • Xia Wang, Prashant Mani, Debi P. Sarkar, Namita Roy-Chowdhury, Jayanta Roy-Chowdhury
    Pages 117-139

  13. Sources of Adult Hepatic Stem Cells: Haematopoietic

    • Rosemary Jeffery, Richard Poulsom, Malcolm R. Alison
    Pages 141-154

  14. Production of Hepatocyte-Like Cells from Human Amnion

    • Toshio Miki, Fabio Marongiu, Ewa C.S. Ellis, Ken Dorko, Keitaro Mitamura, Aarati Ranade et al.
    Pages 155-168

  15. Generation of Hepatocytes from Human Embryonic Stem Cells

    • Niloufar Safinia, Stephen L Minger
    Pages 169-180

  16. Isolation, In Vitro Cultivation and Characterisation of Foetal Liver Cells

    • Yue Wu, Chetan C. Shatapathy, Stephen L. Minger
    Pages 181-191

  17. Human Intrahepatic Biliary Epithelial Cell Lineages: Studies In Vitro

    • Ruth Joplin, Stivelia Kachilele
    Pages 193-206

  18. Liver Cell Labelling with MRI Contrast Agents

    • Michel Modo, Thomas J. Meade, Ragai R. Mitry
    Pages 207-219

  19. Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory

    • Sharon C. Lehec
    Pages 221-227

  20. Back Matter

    Pages 229-231
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Keywords

About this book

Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.

Editors and Affiliations

  • King’s College Hospital, London, UK

    Anil Dhawan

  • School of Medicine, King’s College London, London, UK

    Robin D. Hughes

Bibliographic Information

  • Book Title: Hepatocyte Transplantation

  • Book Subtitle: Methods and Protocols

  • Editors: Anil Dhawan, Robin D. Hughes

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-201-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-883-6Published: 28 January 2009

  • eBook ISBN: 978-1-59745-201-4Published: 19 December 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 231

  • Topics: Biochemistry, general, Cell Biology, Stem Cells

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