Overview
- Features methods involving a wide variety of tissue types in one convenient volume
- Written by leading experts in an easy-to-use step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences
- Covers protocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 633)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
Reviews
From the reviews:
“This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. … This book is recommended for researchers and students who are interested in studying the culture of cells from animal models.” (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)Editors and Affiliations
Bibliographic Information
Book Title: Mouse Cell Culture
Book Subtitle: Methods and Protocols
Editors: Andrew Ward, David Tosh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-019-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-58829-772-3Published: 11 March 2010
Softcover ISBN: 978-1-4939-6086-6Published: 23 August 2016
eBook ISBN: 978-1-59745-019-5Published: 10 March 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 257
Topics: Cell Biology, Biochemistry, general, Cell Culture, Animal Models