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Stress Responses

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Christine M. Oslowski

    1. Wentworth Institute of Technology, Boston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1292)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Assays for Detecting and Characterizing a Variety of Cellular Processes Under Cell Stress

    1. Front Matter

      Pages 1-1
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    2. Methods for Studying ER Stress and UPR Markers in Human Cells

      • Donna Kennedy, Afshin Samali, Richard Jäger
      Pages 3-18

    3. Assays for Induction of the Unfolded Protein Response and Selective Activation of the Three Major Pathways

      • Ananya Gupta, Danielle E. Read, Sanjeev Gupta
      Pages 19-38

    4. Assays to Characterize Molecular Chaperone Function In Vitro

      • Martin Haslbeck, Johannes Buchner
      Pages 39-51

    5. Analysis of the Heat Shock Factor Complex in Mammalian HSP70 Promoter

      • Mitsuaki Fujimoto, Ryosuke Takii, Naoki Hayashida, Akira Nakai
      Pages 53-65

    6. Immunofluorescence-Based Methods to Monitor DNA End Resection

      • Bipasha Mukherjee, Nozomi Tomimatsu, Sandeep Burma
      Pages 67-75

    7. Visualizing the Spatiotemporal Dynamics of DNA Damage in Budding Yeast

      • Chihiro Horigome, Vincent Dion, Andrew Seeber, Lutz R. Gehlen, Susan M. Gasser
      Pages 77-96

    8. Detecting Reactive Oxygen Species by Immunohistochemistry

      • Geou-Yarh Liou, Peter Storz
      Pages 97-104

    9. Investigating Inflammasome Activation Under Conditions of Cellular Stress and Injury

      • Clare C. Cunningham, Emma M. Corr, Donal J. Cox, Aisling Dunne
      Pages 105-113

    10. Methods for Studying microRNA Functions During Stress

      • Yoshinari Ando, Anthony K. L. Leung
      Pages 115-128

    11. Measuring Autophagy in Stressed Cells

      • Marina N. Sharifi, Erin E. Mowers, Lauren E. Drake, Kay F. Macleod
      Pages 129-150

    12. Detection of Apoptosis Using Fluorescent Probes

      • Grishma Khanal, Himali Somaweera, Meicong Dong, Todd Germain, Megan Ansari, Dimitri Pappas
      Pages 151-161

  3. Studying Cell Stress in the Context of Disease

    1. Front Matter

      Pages 163-163
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    2. Measuring Death of Pancreatic Beta Cells in Response to Stress and Cytotoxic T Cells

      • Jibran A. Wali, Prerak Trivedi, Thomas W. Kay, Helen E. Thomas
      Pages 165-176

    3. Adaptation of the Secretory Pathway in Cancer Through IRE1 Signaling

      • Stéphanie Lhomond, Nestor Pallares, Kim Barroso, Kathleen Schmit, Nicolas Dejeans, Hélèna Fazli et al.
      Pages 177-194

    4. Studying Nitrosative Stress in Parkinson’s Disease

      • Kenny K. K. Chung
      Pages 195-201

  4. Perspectives

    1. Front Matter

      Pages 203-203
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    2. Cross Talk Between ER Stress, Oxidative Stress, and Inflammation in Health and Disease

      • Aditya Dandekar, Roberto Mendez, Kezhong Zhang
      Pages 205-214

    3. Stress Responses During Ageing: Molecular Pathways Regulating Protein Homeostasis

      • Emmanouil Kyriakakis, Andrea Princz, Nektarios Tavernarakis
      Pages 215-234
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Keywords

About this book

This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases. In addition, this book reviews the crosstalk between different stress pathways, stress responses during ageing, and targeting stress for regenerative medicine. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and instructive, Stress Responses: Methods and Protocols seeks to aid scientists to further study multiple stress pathways and outcomes triggered by such stresses. In depth knowledge of cellular stress will eventually lead to the development of novel therapeutics to prevent or treat related diseases.

Editors and Affiliations

  • Wentworth Institute of Technology, Boston, USA

    Christine M. Oslowski

About the editor

Christine M. Oslowski, Ph.D. 21 Franklin Street, Apt 7 Somerville, MA 02145 Cell: (617) 501-6711 Email: coslowsk@bu.edu

Bibliographic Information

  • Book Title: Stress Responses

  • Book Subtitle: Methods and Protocols

  • Editors: Christine M. Oslowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2522-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2521-6Published: 25 March 2015

  • Softcover ISBN: 978-1-4939-4848-2Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2522-3Published: 24 March 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 246

  • Number of Illustrations: 23 b/w illustrations, 28 illustrations in colour

  • Topics: Cell Biology

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