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  • Book
  • © 2015

Tissue Morphogenesis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1189)

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Softcover Book USD 119.99
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Hardcover Book USD 169.99
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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
  2. Imaging Morphogenesis

    1. Front Matter

      Pages 1-1
    2. Hemodynamic Flow Visualization of Early Embryonic Great Vessels Using μPIV

      • Selda Goktas, Chia-Yuan Chen, William J. Kowalski, Kerem Pekkan
      Pages 17-30
    3. Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis

      • Jacky G. Goetz, Fabien Monduc, Yannick Schwab, Julien Vermot
      Pages 31-46
    4. Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis

      • Chelsea L. Gregg, Andrew K. Recknagel, Jonathan T. Butcher
      Pages 47-61
    5. Light Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly

      • Khaled Khairy, William C. Lemon, Fernando Amat, Philipp J. Keller
      Pages 79-97
    6. Active Cell and ECM Movements During Development

      • Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, András Czirók
      Pages 123-132
  3. Culture Models of Morphogenesis

    1. Front Matter

      Pages 133-133
    2. 3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion

      • Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, Andrew J. Ewald
      Pages 135-162
    3. Culture of Mouse Embryonic Foregut Explants

      • Felicia Chen, Wellington V. Cardoso
      Pages 163-169
    4. Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues

      • Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, Celeste M. Nelson
      Pages 191-206
  4. Manipulating Cells and Tissues In Vivo

    1. Front Matter

      Pages 207-207
    2. UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo In Vivo and Ex Vivo

      • Michael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, Carl-Philipp Heisenberg
      Pages 219-235

About this book

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

Editors and Affiliations

  • Chemical & Biological Engineering, Princeton University, USA

    Celeste M. Nelson

Bibliographic Information

  • Book Title: Tissue Morphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Celeste M. Nelson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1164-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1163-9Published: 23 September 2014

  • Softcover ISBN: 978-1-4939-4585-6Published: 30 April 2017

  • eBook ISBN: 978-1-4939-1164-6Published: 22 September 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 351

  • Number of Illustrations: 30 b/w illustrations, 76 illustrations in colour

  • Topics: Cell Biology, Gene Function

Buy it now

Buying options

Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access