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Life Sciences - Cell Biology | Stem Cell Transcriptional Networks - Methods and Protocols

Stem Cell Transcriptional Networks

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 1150

Kidder, Benjamin L (Ed.)

2014, X, 286 p. 150 illus., 142 illus. in color.

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  • Includes cutting-edge methods and protocols for the interrogation of stem cell transcriptional networks
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks.

Content Level » Professional/practitioner

Keywords » Cellular expression - Epigenetic landscapes - Next-generation sequencing - Protein-DNA binding interactions - Regenerative medicine - Stem cell biology - Transcriptional programs

Related subjects » Cell Biology - Life Sciences

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