Editors:
- Includes cutting-edge methods and protocols for the interrogation of stem cell transcriptional networks
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1150)
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Table of contents (19 protocols)
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Front Matter
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Next-Generation Sequencing Library Preparation and Data Analysis
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Front Matter
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Visual Analysis and Interpretation of Large-Scale Interaction Networks
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Front Matter
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Transcriptional Networks in Embryonic and Adult Stem Cell
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Front Matter
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Embryo Culture and Derivation of Stem Cells
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Front Matter
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Transcriptional Programs that Promote Self-Renewal, Reprogramming, and Transdifferentiation
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Front Matter
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About this book
Stem Cell Transcriptional Networks: Methods and Protocols collects techniques used to increase our understanding of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. The volume opens with a section on next-generation sequencing library preparation and data analysis. Continuing with a collection of protocols on visual analysis and interpretation of large-scale interaction networks, this detailed compilation features transcriptional networks in embryonic and adult stem cells, embryo culture and derivation of stem cells, as well as transcriptional programs that promote self-renewal, reprogramming, and transdifferentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Transcriptional Networks: Methods and Protocols aims to provide a key resource for biologists seeking to interrogate these vital networks.
Editors and Affiliations
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Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, USA
Benjamin L. Kidder
Bibliographic Information
Book Title: Stem Cell Transcriptional Networks
Book Subtitle: Methods and Protocols
Editors: Benjamin L. Kidder
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-0512-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2014
Hardcover ISBN: 978-1-4939-0511-9Published: 18 April 2014
Softcover ISBN: 978-1-4939-5406-3Published: 23 August 2016
eBook ISBN: 978-1-4939-0512-6Published: 17 April 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 286
Number of Illustrations: 8 b/w illustrations, 142 illustrations in colour
Topics: Cell Biology, Stem Cells