Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells, but also to ascertain their abilities to be used in industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods.
This volume gives the readers a complete review of present state-of-the-art in Japan and other countries where this field is well advanced. The Proceedings will be useful for the cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working in either academic environments or in industries of biotechnology and pharmacy.
Plenary Lecture. Recombinant expression of human collagens; J. Myllyharju.
Symposium: Recent Advances in the Materials and Reconstructive Therapy and Tissue Engineering. Expression profiling of parietal endoderm cells and development of systems for large-scale production of laminins; Y. Hayashi, et al. Synthetic peptides from laminin for tissue engineering; M. Mochizuki, M. Nomizu. A novel technology for conversion of in vivo tissue architecture into a three-dimensional in vitro culture mode; T. Takezawa. Construction of liver model with genetically engineered human HepG2 cells; T. Omasa, S. Enosawa.
Structural Biology and Signal Transduction of Animal Cells. Determinants of ligand specificity in groups I and IV WW domains; Y. Kato, et al. Role of the WW domain in signaling and disease; M. Sudol. Structural biology of signal transduction mediated by Grb2; F. Inagaki.
Cell Regulatory Functions of Food Factors and Natural Resources with Special Reference to Health Promotion and Disease Prevention. Modulation of leukocyte function by food factors; M. Yamazaki, H. Ueda. Anticancer effects of lignans; N. Saarinen, et al. Effect of sulfur-containing active principles in garlic on the physiological function of hepatocytes; L.Y. Sheen. Suppression of free radical-induced mutation of animal cell genes by food factors; A. Murakami, et al.
Glycoengineering in Animal Cell Technology. Chemoenzymatic approaches for the preparation of oligosaccharides and glycopeptides; K. Ajisaka.
ESACT Special Lecture: Production of Biologicals. Cell growth arrest by nucleotides as a tool for improved production of biopharmaceuticals; A.V. Carvalhal, et al.
Cell Culture Engineering. Transient gene expression: A novel mammalian cell-based technology for recombinant protein production; F.M. Wurm, et al.
Gene Therapy. Optimization of the production of retroviral vectors: Influences of the sugar source; B. Gény-Fiamma, et al.
Session: Cell Culture Engineering. Effects of glucose and CO2 concentrations on CHO cell physiology; S. Takuma, et al. Suppression of glutamate-induced neural cell death by electrolyzed-reduced water; T. Kashiwagi, et al. Development of apoptosis-resistant dihydrofolate reductase-deficient Chinese hamster ovary cell line; S.K. Lee, G.M. Lee. High expression of human monoclonal antibodies in CHO cells selected using a cycloheximide resistance marker; M. Tsukahara, et al. Development of a serum-free medium supplemented with hydrolysate for the suspension culture of recombinant CHO cells producing human thrombopoietin; Y.H. Sung, G.M. Lee. Effect of growth factors and ascorbic acid 2-phosphate on liver specific functions of porcine hepatocytes; A. Okamura, et al. Culture of porcine hepatocytes using radial flow bioreactor system; Y. Shiba, et al. The role of glutamine in hybridoma cell culture; H. Matsuoka, et al. 8 Additional Articles.
Production of Biologicals. An engineering method to high yielding production of recombinant anti-HIV monoclonal antibody using generic multi feed fermentation; K. Kawatsu, et al. Proliferation and monoclonal antibody production of hybridoma cells immobilized on non-woven polyester fabric disks; E. Aslankaraoglu, et al. Mass production in yeast of laminins as the material of reconstructive therapy; K. Mizuno, et al.
Functional Cell Lines. Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line A549; A.T. Koparal, M. Zeytinoglu. Establishment of dendritic cell line from caspase-1-deficient mice; B. Nowak, et al. Isolation and characterization of fetal bovine cells derived from liver and bone marrow; T. Takenouchi, T. Takezawa. Artificial selection of high proliferating and apoptosis-resistant NS0 cells for industrial production systems; D. Chau, M. Al-Rubeai.
Immunologicals, Monoclonal Antibodies and Vaccines. Endothelial cells culture and its use to produce a vaccine against rickettsiae; V. Hedrick, et al. Monocytes suppress immune responses of peripheral blood lymphocytes: Possible implication of immature dendritic cells; M. Yamashita, et al. Establishment and analysis of germ-free T cell receptor transgenic mice; M. Fujioka, et al. Development of a novel process to produce a veterinary rabies vaccine in BHK-21 cells grown on microcarriers in a 20 liter bioreactor; S. Rourou, et al. Epitope-specific suppression of immune response against ß-lactoglobulin induced by oral administration of cow's milk with heat treatments; A. Enomoto, et al. 3 Additional Articles.
Transplantation, Artificial Organs, and Organ Substitutes. Evaluation of the immuno-protective effects of the new-type of bags using ELISA and FACS analysis; M.S. Rahman, et al. Effect of silk protein sericin on hepatoblastoma HepG2 cells; T. Kumagai, et al. Isolation of human mesenchymal stem cell-like fibroblast from adipose tissue for reconstructive therapy; T Miyazaki, et al. Change of the cellular function by connexin gene transfection in a hepatoma cell line; J. Yang, et al.
Transgenic Animals. Production of pharmaceutical proteins by transgenic avian; A. Hotta, et al.
Gene Therapy. Influence of serum concentration on cell growth and retrovirus production and decay kinetics; J.N. Warnock, M. Al-Rubeai.
Cell Regulatory Factors and Signal Transduction. Neural precursor cells from the adult mouse cerebral cortex; M. Matsumura, et al. The inhibition of MMP-2 expression in human fibrosarcoma HT 1080 cells by electrolyzed-reduced water; J. Ye, et al. The role of plasminogen in the proliferation of murine hepatocytes in primary culture; M. Akao, et al. Transcriptional regulation mechanisms of telomerase reverse transcriptase gene responsible for ubiquitous expression of telomerase in mouse; T. Fujiki, et al. Cell proliferation switch responsive to fluorescein dimer; H. Kimura, et al. Hydrogen peroxide triggers cellular senescence programs: Possible application to tumor suppression; K. Yoshizaki, et al. Essential role of cell-cell interaction in primary hepatocyte cultures; T. Dohda, et al. 4 Additional Articles.
Functional Substances in Food and Natural Sources. The suppressive effect of electrolyzed reduced water on lipid peroxidation; T. Hamasaki, et al. Electrolyzed reduced water induces differentiation of K-562 human leukemia cells; T. Komatsu, et al. Oral administration of soy isoflavones and determination of serum isoflavone levels in Sprague-Dawley rats; S. Yasuda, et al. Effect of ß-casein on cytokine production of mouse spleen lymphocytes; T. Okamoto, et al. Changes in the relevant test parameters of 219 diabetes patients under the influence of the so-called "Nordenau-Phenomenon" in the prospective observation procedure; Z. Gadek, et al. Decreased intracellular peroxide levels of rat ascites hepatoma cells resveratrol and sera from resveratrol-treated rats; D. Miura, et al. Inhibitory effects of several flavonoids on prostaglandin production and E-selectin expression in cultured animal cells; Y. Takano-Ishikawa, et al. 8 Additional Articles.
Animal Cells for in vitro Assay. Effects of carvacrol on sister chromatid exchanges in human lymphocyte cultures; E. Ipek, et al. Biocompatible biomaterials for the human chrondrocyte differentiation estimated by RT-PCR method; T. Tsuchiya, et al. Different expression of gap junctional protein connexin 43 in two strains of mice after one-month implantation of poly-L-lactic acid; S. Ahmed, T. Tsuchiya. Changes on cell surface and microfilament distribution during myoblast differentiation and transformation in vitro; M. Zeytinoglu, H.Y. Ocak. Primary cilium in CO25 cell line; M. Zeytinoglu, H.Y. Ocak. Environmental chemicals affect nerve cell differentiation in SFME cells through specific gene expression; Y. Fuke, et al. Evaluation of the cornea cells affected by multi-purpose solutions for contact-lens; J. Park, T. Tsuchiya.
Author Index. Subject Index.