Proceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002 Košice, Slovakia - 25-26 April 2013
de Almeida, A., Eckersall, D., Bencurova, E., Dolinska, S., Mlynarcik, P., Vincova, M., Bhide, M. (Eds.)
2013, Approx. 305 p. 64 illus., 21 illus. in color.
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Reflects the will of a group of scientists that merge innovation with excellence of research
Of interest to scientists at the early stages of their careers as well as to researchers well established in the field and to whom proteomics may be the necessary next step towards more in-depth research activities
A witness to the vitality of the area and the importance it holds to animal and food research, to science, industry, government agencies, the consumer and ultimately the society as a whole
Proteomics may be defined as the large-scale study of the proteome, i.e. a set of proteins being expressed in a certain fluid, tissue, organ or organism. Although still of limited and restricted use in most areas of farm animal and veterinary research, proteomics potential is unequivocal holding a significant promise in applications such as vaccine and drug development, animal product quality, physiology or toxicology. Nevertheless, proteomics use has been growing steadily during the last 2-3 years and, as time goes by; proteomics-based studies are more and more common, not just to scientists but to the general public, unraveling their full potential. This book reflects the will of a group of scientists that merge innovation with excellence of research and to whom the dissemination of knowledge and innovation through cooperation is a key essential point. It will be of interest to scientists at the early stages of their careers as well as to researchers well established in the field and to whom proteomics may be the necessary next step towards more in-depth research activities. By providing a collection of diverse scientific interests, Farm Animal Proteomics 2013 is also a witness to the vitality of the area and the importance it holds to animal and food research, to science, industry, government agencies, the consumer and ultimately the society as a whole.
The Cost Organisation.- Farm animal proteomics: going to the European capital of culture 2013; Mangesh Bhide.- Part I – Invited plenary communications.- Mining deeper into the proteome: pros and cons of pre-fractionation and depletion; Ingrid Miller.- Detection and annotation of common post-translational modifications in mass spectrometry data; Julien Mariethoz et al.- Applied bioinformatics in the structural, post-genomic era; Dimitrios Vlachakis et al.- PTMomics – a potpourri of experimental approaches; Ana V. Coelho.- Gel-free quantitative proteomics approaches, current status; Gabriel Mazzucchelli, Edwin De Pauw.- New proteomics strategies applied to clinical studies; Bruno Domon, Sebastien Gallien.- Neuro-immune proteomic crosstalk in health and disease: partners in love, partners in divorce; Norbert Zilka et al.- Top-down proteomics: 2D gels are an integral part of the process; Jens R. Coorssen.- MALDI imaging mass spectrometry: applications, limitations and potential; Charles Pineau.- Parasite- and host-derived proteins involved in African trypanosome brain invasion and dysfunction; Krister Kristensson.- Proteomics at the host: pathogen interface; Richard Burchmore.- Microbial proteomics in food safety and animal welfare; Paola Roncada et al.- The search of stress markers in porcine by using proteomics; Anna Marco-Ramell et al.- Quest for biomarkers of the lean-to-fat ratio by proteomics in beef production; Muriel Bonnet et al.- The LEXSY platform for recombinant protein expression; Reinhard Breitling.- Part II – Advancing methodology for farm animal proteomics and bioinformatics.- Data-independent acquisition strategies for quantitative proteomics; Ute Distler et al.- Small intestinal response to enterotoxigenic Escherichia coli infection in pigs as revealed by label free UPLC/MSE proteomics; Laura Soler et al.- Rapid protein production of Atlantic Salmon, Salmo salar, serum amyloid A (SAA) in an inducible Leishmania tarentolae expression system; Mark Braceland et al.- Proteomic profiling of cerebrospinal fluid in canine degenerative myelopathy; Viviana Greco et al.- Automatic prediction of PTMs in Ehrlichia ruminantium – creating new datasets for Quickmod analyses; Miguel Ventosa et al.- Tandem mass spectrometry for species recognition and phenotyping in fish; Tune Wulff et al.- MALDI imaging mass spectrometry of ageing and osteoarthritic cartilage; Mandy J. Peffers et al.- Protein expression in bovine mononuclear cells after stimulation with lipopolysaccharides and lipoteichoic acid: a proteomic approach; Laura Restelli et al.- The ‘hidden’ proteome of cow’s and Jennys’ milk as revealed by combinatorial peptide ligand libraries; Elisa Fasoli et al.- Comparison between Coomassie Blue and Silver staining in porcine saliva samples for proteomics: technical considerations: a preliminary experiment; Maria Fuentes-Rubio et al.- Isoelectric focusing in characterisation of alkaline phosphatase isozyme from bovine nasal secretion and mucosa; M. Faizal Ghazali et al.- Part III – Infectious diseases.- PilE4 may contributes in the adhesion of Francisella to brain microvascular endothelial cells; Elena Bencurova et al.- Comparative proteomics analysis of campylobacteriosis in human and swine: a study of intestine epithelial cell response to bacterial infection; Carmen Aguilar et al.- Identification of amino acid residues of OspA of Borrelia involved in binding to CD40 receptor; Patrik Mlynarcik et al.- Omics approaches to study the Rickettsia Ehrlichia ruminantium: towards improved knowledge on Heartwater disease; Isabel Marcelino et al.- Detection of Salmonella antigens expressed in swine gut by hydrophobic antigen tissue triton extraction (HATTREX); Rodrigo P. Martins et al.- New approaches in the diagnosis and treatment of endotoxemia in calves: proteomic investigation and effects of intravenous choline administration; Meric Kocaturk et al.- Changes on bovine aorta endothelial cells (BAE) proteome upon infection with the rickettsia Ehrlichia ruminantium; Elisabete Pires et al.- Effect of Staphylococcus aureus infection on fibrinogen and plasma protein concentrations in experimentally obese rabbits; E. Dishlyanova et al.- Trypanosoma brucei brucei binds human complement regulatory protein C4BP; Saskia Dolinska et al.- A proteomic analysis of canine serum during the course of babesiosis; Josipa Kuleš et al.- The acute phase reaction in goats after experimentally induced E. coli mastitis: a proteomic approach; Lazarin Lazarov et al.- The effect of microbial challenge on the intestinal proteome of broiler chickens; Emily L. O’Reilly et al.- 2D DIGE comparative analysis of Escherichia coli strains with induced resistance to enrofloxacin; Cristian Piraset al.- In situ assessment of differential adhesion of neuroinvasive and non-neuroinvasive Borrelia strains to BMECs; Lucia Pulzova et al.- Caecal IEL, blood lymphocytes and intestinal mucin study in chickens after probiotic prevention and S. Enteritidis infection; Viera Revajova et al.- Bovine anaplasmosis: IgG2 reactivity in vaccinated cattle against conserved recombinant outer membrane proteins; Theah Molad et al.- Identification BMECs receptors interacting with Trypanosoma brucei brucei; Miroslava Vincova et al.- Effect of short-time overweight gain on the plasma PON1 activity after acute Staphylococcus aureus infection in rabbits ; Tatyana I. Vlaykova et al.- Part IV – Animal production.- Biomarkers of winter disease in gilthead seabream: a proteomics approach; Denise Schrama et al.- The absorption of colostral proteins in newborn lambs: an iTRAQ proteomics study; Lorenzo E. Hernandez-Castellano et al.- Plasma proteome profiles predict diet-induced metabolic syndrome and the early onset of metabolic syndrome in a pig model; Marinus F.W. te Pas et al.- Changes in protein abundance in the vertebral column of Atlantic salmon (Salmosalar) fed variable dietary P levels; Eva Veiseth-Kent et al.- Neurotransmitter levels and proteomic approach in pig brain: pre-slaughter handling stress and cognitive biases; Laura Arroyo et al.- The sheep (Ovis aries) mammary gland mitochondrial complexes: establishment of a Blue-Native PAGE separation method as a model for other ruminant species; Carlos Filipe et al.- Bitter taste in water-buffalo (Bubalus bubalis): from T2R gene identification to expression studies; Ana M. Ferreira et al.- Seasonal weight loss in dairy goats from the Canary Islands: towards an integrated omics approach?; Joana R. Lerias et al.- Analysis of endogenous and recombinant bovine somatotropin in serum; Marco H. Blokland et al.- Investigation of salivary acute phase proteins in calves; Mizanur Rahman et al.- Targeted proteomics as a tool for porcine acute phase proteins measurements; Anna Marco-Ramell et al.- LC-MS/MS analyses of visceral and subcutaneous adipose tissue proteomes in goats; Laura Restelli et al.- Comparison of the patterns of milk serum proteins in subclinical mastitis caused by four common pathogens in dairy cows; Shahabeddin Safi et al.- Intrauterine treated lambs as a model for the study of Intrauterine Growth Restriction (IUGR) and consequent metabolic disorders; Tommaso Serchi et al.- Effect of estradiol on biochemical bone metabolism markers in dairy cows; Jože Starič et al.- Dynamics of metabolic and oxidative stress parameters in dairy heifers during transition period; Petra Zrimšek et al.- The proteomics of wool follicles; Jeffrey E. Plowman et al.- Relationships between mediators of the acute phase response and altered energetic metabolism in dairy cows after calving; Gabriel Kovač et al.- Serum protein electrophoretic pattern in clinically healthy calves and cows; Csilla Tothova et al.- Part V – Food safety quality.- A mass spectrometric scoring system for oxidative damage in dairy foods; Stefan Clerens et al.- Shotgun proteomics in blue mussels exposed to benthic trawler-induced sediment resuspension from a polluted fjord; Sara Tedesco et al.- Milk and cheese microbiome for safety and quality of dairy products; Alessio Soggiu et al.- Molecular characterization of Maltese honey: diastase and proline levels changes in Maltese honey seasons; Adrian Bugeja Douglas et al.- Untargeted metabolomic analyses open new scenarios in post mortem pig muscles: Casertana and Large White; Cristina Marrocco et al.- The role of salt in dry cured ham processing characterized by LC-MS/MS-based proteomics; Gianluca Paredi et al.- Proteolytic action of caspases 3 and 7 on the hydrolysis of bovine and porcine muscle myofibrillar proteins; Rosa A. Rodriguez-Frometa et al.- Allergen characterization in Mediterranean farmed fish: a proteomic approach; I.M. Luis et al.- Protein exudation from salted meat in processing by cold tumbling; Priit Soosaar et al.- Peptidomics in extended shelf life dairy products, for assessment of proteolysis and product quality; Nanna Stengaard Villumsen et al.- Egg proteome: the protein and carotenoids characteristics deriving from laying hens reared in different housing systems and under different environmental conditions; Charles Spiteri et al.- Proteomics and transcriptomics investigation on longissimus muscles in Large White and Casertana pig breeds; Anna Maria Timperio et al.- Acknowledgements.