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Electron Probe Microanalysis

Applications in Biology and Medicine

  • Conference proceedings
  • © 1989

Overview

Editors:
  1. Karl Zierold

    1. Max-Planck-Institut für Systemphysiologie, Dortmund 1, Germany

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  2. Herbert K. Hagler

    1. Department of Pathology, University of Texas Southwestern Medical Center, Dallas, USA

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Springer Series in Biophysics (BIOPHYSICS, volume 4)

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Table of contents (23 papers)

  1. Front Matter

    Pages I-XVI
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  2. The History of Electron Probe Microanalysis in Biology

    1. The History of Electron Probe Microanalysis in Biology

      • T. A. Hall
      Pages 1-15

  4. Analytical Techniques

  5. Biological Applications

    1. Intracellular element localization

      1. Application of X-Ray Microanalysis and Electron Energy Loss Spectroscopy to Studies of Secretory Cell Biology
        • Richard L. Ornberg, Gemma A. J. Kuijpers
        Pages 153-168
      2. X-Ray Microanalysis of Freshly Isolated Cells in Suspension
        • Alice Warley
        Pages 169-179
      3. X-Ray Microanalysis and Free Calcium Measurements in Cultured Neonatal Rat Ventricular Myocytes
        • Herbert K. Hagler, Alan C. Morris, L. Maximilian Buja
        Pages 181-197

    3. Dynamic processes

      1. Quantitative X-Ray Elemental Mapping of Dynamic Physiologic Events in Skeletal Muscle
        • Peter Ingram, Rashid Nassar, Ann LeFurgey, Scott Davilla, Joachim R. Sommer
        Pages 251-264
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Keywords

About this book

The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti­ cular diffusible ions (elements). 2. Electron probe microanaly­ sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con­ verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo­ gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.

Editors and Affiliations

  • Max-Planck-Institut für Systemphysiologie, Dortmund 1, Germany

    Karl Zierold

  • Department of Pathology, University of Texas Southwestern Medical Center, Dallas, USA

    Herbert K. Hagler

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