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Basic Methods for the Biochemical Lab presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables.
The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates); electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis); chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC); immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA); centrifugation (differential and density gradient centrifugation for cells and cellular fractions); radioactivity (labeling and counting); and buffers (buffer properties and compositions).
In additional chapters, tables provide speedy access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed.