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Life Sciences - Biochemistry & Biophysics | Calcium Signaling Protocols

Calcium Signaling Protocols

Series: Methods in Molecular Biology, Vol. 937

Lambert, David G., Rainbow, Richard D. (Eds.)

3rd ed. 2013, XI, 360 p. 51 illus., 17 illus. in color.

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  • Combines brand new protocols with heavily revised and improved methodologies
  • Features lab-ready procedures with step-by-step instruction
  • Includes expert tips and key implementation advice to ensure successful results

The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.

Content Level » Professional/practitioner

Keywords » Calcium signaling - Channel activity measurement - Intracellular Ca2+ - Regulation - Specialist measurement systems - Store release - Suspension-based fluorimetric assay

Related subjects » Biochemistry & Biophysics

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