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Life Sciences - Biochemistry & Biophysics | Clinical Applications of Capillary Electrophoresis - Methods and Protocols

Clinical Applications of Capillary Electrophoresis

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 919

Phillips, Terry M., Kalish, Heather (Eds.)

2013, XIII, 311 p. 70 illus., 28 illus. in color.

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used.  Clinical Applications of Capillary Electrophoresis aims to give an in-depth manual of CE applications in several important areas of clinical science.  Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Clinical Applications of Capillary Electrophoresis seeks to serve as a valuable source of information not only for clinical pathologists, but also clinical scientists who wish to apply the technique to diagnosis and research.

Content Level » Professional/practitioner

Keywords » bacteriology - capillary electrophoresis - clinical chemistry - disease biomarker discovery - hematology - immunology - virology

Related subjects » Biochemistry & Biophysics

Table of contents 

Part I. Overview

 

1. An Overview of CE in Clinical Analysis

            David S. Hage

 

Part II. Applications in Clinical Chemistry

 

2. Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection

            Petr Tůma and Karel Štulik

 

3. The Selective Determination of Sulfates, Sulfonates and Phosphates in Urine by Capillary Electrophoresis – Mass Spectrometry

            Svenja-Catharina Bunz, and Christian Neusüß

 

4. Analysis of Neurotransmitter Amino Acids by CE-LIF Detection in Biological Fluids

            Angelo Zinellu, Salvatore Sotgia, Luca Deiana, and Ciriaco Carru

 

5. Expanded Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)

            Philip Britz-McKibbin

 

6. Development of an |On-Site Measurement System for Salivary Stress-Related Substances Based on Microchip CE

            Yoshihide Tanaka and Nahoko Naruishi

 

7. Simultaneous Determination of Atenolol and Amiloride by CZE with Capacitively Coupled Conductivity Detection (C4D)

            Khaldun M. AL Azzam and Hassan Y. Aboul-Enein

 

8. A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Detection

            Shulin Zhao and Yi-Ming Liu

 

9. High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems

            Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert

 

10. Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detection

            Xin Hai, Erwin Adams, and Ann Van Schepdael

 

Part III. Applications in Hematology

 

11. ABO Genotyping by Capillary Electrophoresis

            James Chun-I Lee, Hsing-Mei Hsieh, Hsiao-Feng Teng, and Li-Chin Tsai

 

12. Separation of Hemoglobin Variants by Capillary Electrophoresis

            Frédéric Cotton and Béatric Gulbis

 

13. Newborn Screening for Hemoglobinopathies Using Capillary Electrophoresis

            P.C. Giordano

 

Part IV. Applications in Bacteriology and Virology

 

14. Application of CZE in the Differentiation of Staphylococcus Aureus Strains

            Bogusław Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz

 

15. Multiplex and Quantitative Pathogen Detection with High-Resolution Capillary Electrophoresis-Based Single Strand Conformation Polymorphism

            Hee Sung Hwang, Gi Won Shin, Boram Chung, Jeongkyeong Na, and Gyoo Yeol Jung

 

16. Application of CGE to Virus Identification

            Julia A. Fruetel and Victoria A. VanderNoot

 

Part V. Applications in Disease Biomarker Discovery

 

17. Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery

            Xueping Fang, Chenchen Wang, and Cheng S. Lee

 

18. Integrated Affinity and Electrophoresis Systems for Multiplexed Biomarker Analysis

            Pamela N. Nge, Jayson V. Pagaduan, Weichun Yang, and Adam T. Woolley

 

19. Urinary Proteomics Based on Capillary Electrophoresis Coupled to Mass Spectrometry in Kidney Disease

            Amaya Albalat, Julia Franke, Julien Gonzalez, Harald Mischak, and Petra Zürbig

 

20. Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as Atherothrombosis Biomarker

            Angel Puerta, Pedro J. Martin-Alvarez, Sara Ongay, Jose C. Diez-Masa and  Mercedes de Frutos

 

Part VI. Applications in Immunology

 

21. Chip-Based Immunoassays

            Akwasi A. Apori and Amy E. Herr

 

22. CE Analysis of g-Globulin Mobility and Potential Clinical Utility      

            Dieter Vanderschaeghe, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe

 

23. Cytokine Analysis by Immunoaffinity Capillary Electrophoresis

            Mark Mendonca and Heather Kalish

 

Part VII. Applications in Clinical Genetic Analysis

 

24. Capillary Electrophoresis for the Detection of Fragile X Expanded Alleles

            Rong Mao, Pinar Bayrak-Toydemir, and Elaine Lyon

 

25. Analysis of Microsatellite Instability by Microfluidic-Based Electrophoresis

            Natalia Elfimova, Wafa Amer, and Margarete Odenthal

 

26. HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease

            Ewa H. Lavant and Joyce Carlson

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