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Unnatural Amino Acids

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Loredano Pollegioni

    1. Dipto. Biotecnologie e di Scienze Mol, Molecolari (DBSM), Università degli Studi dell'Insubria, Varese, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Stefano Servi

    1. Dipto. Chimica, Politecnico di Milano, Milano, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step readily reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 794)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Synthesis of Unnatural Amino Acids

    1. Front Matter

      Pages 1-1
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    2. Preparation of Unnatural Amino Acids with Ammonia-Lyases and 2,3-Aminomutases

      • László Poppe, Csaba Paizs, Klaudia Kovács, Florin-Dan Irimie, Beáta Vértessy
      Pages 3-19

    3. Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids

      • Paola D’Arrigo, Davide Tessaro
      Pages 21-35

    4. Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates

      • Gwynneth F. Matcher, Rosemary A. Dorrington, Stephanie G. Burton
      Pages 37-54

    5. Preparation of Glutamate Analogues by Enzymatic Transamination

      • Thierry Gefflaut, Zeinab Assaf, Martine Sancelme
      Pages 55-72

    6. Carbon–Carbon Bond-Forming Enzymes for the Synthesis of Non-natural Amino Acids

      • Pere Clapés, Jesús Joglar, Mariana Gutiérrez
      Pages 73-85

    7. Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis

      • Francisco Javier Las Heras-Vázquez, Josefa María Clemente-Jiménez, Sergio Martínez-Rodríguez, Felipe Rodríguez-Vico
      Pages 87-104

  3. Application of Unnatural Amino Acids

    1. Front Matter

      Pages 105-105
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    2. NMR Analysis of Unnatural Amino Acids in Natural Antibiotics

      • Franca Castiglione
      Pages 107-124

    3. Site-Specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes

      • Jennifer C. Peeler, Ryan A. Mehl
      Pages 125-134

    4. Application of Unnatural Amino Acids to the De Novo Design of Selective Antibiotic Peptides

      • Rickey P. Hicks, Amanda L. Russell
      Pages 135-167

    5. Use of Unnatural Amino Acids to Probe Structure–Activity Relationships and Mode-of-Action of Antimicrobial Peptides

      • Alessandro Tossi, Marco Scocchi, Sotir Zahariev, Renato Gennaro
      Pages 169-183

  4. Use of Unnatural Amino Acids in Protein Synthesis

    1. Front Matter

      Pages 185-185
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    2. Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis

      • Jia Liu, T. Ashton Cropp
      Pages 187-197

    3. Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast

      • Qian Wang, Lei Wang
      Pages 199-213

    4. Site-Specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells

      • Nobumasa Hino, Kensaku Sakamoto, Shigeyuki Yokoyama
      Pages 215-228

    5. Incorporation of Unnatural Non-α-Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System

      • Takahiro Hohsaka
      Pages 229-239

    6. Site-Specific Modification of Proteins by the Staudinger-Phosphite Reaction

      • Paul Majkut, Verena Böhrsch, Remigiusz Serwa, Michael Gerrits, Christian P. R. Hackenberger
      Pages 241-249

  5. d-Amino Acids: Analysis and Applications

    1. Front Matter

      Pages 251-251
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Keywords

About this book

Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of  unnatural amino acids in peptides with antimicrobial properties, genetic  incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.

Editors and Affiliations

  • Dipto. Biotecnologie e di Scienze Mol, Molecolari (DBSM), Università degli Studi dell'Insubria, Varese, Italy

    Loredano Pollegioni

  • Dipto. Chimica, Politecnico di Milano, Milano, Italy

    Stefano Servi

Bibliographic Information

  • Book Title: Unnatural Amino Acids

  • Book Subtitle: Methods and Protocols

  • Editors: Loredano Pollegioni, Stefano Servi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-331-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-330-1Published: 29 September 2011

  • Softcover ISBN: 978-1-4939-5888-7Published: 23 August 2016

  • eBook ISBN: 978-1-61779-331-8Published: 30 September 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 409

  • Number of Illustrations: 123 b/w illustrations

  • Topics: Biochemistry, general, Enzymology

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