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Life Sciences - Biochemistry & Biophysics | Molecular Chaperones - Methods and Protocols

Molecular Chaperones

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 787

Calderwood, Stuart K., Prince, Thomas L. (Eds.)

2011, XVIII, 320p. 42 illus..

A product of Humana Press
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  • Approaches the study of heat shock transcription factors both in vivo and in vitro
  • Features step-by-step protocols which are tested and ready for the lab
  • Includes expert tips and vital implementation advice to ensure successful results

The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.  In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.

Content Level » Professional/practitioner

Keywords » HSF proteins - HSP gene promoters - Heat shock proteins - Heat shock response - Heat shock transcription factors - Protein folding - Protein quality control

Related subjects » Biochemistry & Biophysics

Table of contents 

1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

            Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi


2. The Role Of Heat Shock Factors in Stress-Induced Transcription     

            Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood


3. Hsp90 and Client Protein Maturation

            Natalie Wayne, Parul Mishra, and Daniel N. Bolon


4. The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function

            Marc B. Cox and Jill L. Johnson


5. Detecting Hsp90 Phosphorylation

            Mehdi Mollapour and Len Neckers


6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome

            Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan


7. Nucleotide Exchange Factors for Hsp70 Chaperones

            Heike Rampelt, Matthias P. Mayer, and Bernd Bukau


8. Reconstitution of CHIP E3 Ubiquitin Ligase Activity

            Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser


9. Structure-Functions of HspB1 (Hsp27)

            André-Patrick Arrigo


10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene

            Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea


11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities

            Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stéphanie Weidmann, Jean Guzzo, and Carmen Garrido


12. Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA

            H. Elyse Ireland and John H.H. Williams


13. Analysis of Heat Shock Protein Localization Using Flow Cytometry

            Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams


14. Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry

            Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea


15. Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks

            Yunchen Gong, Zhaolei Zhang, and Walid A. Houry


16. Hsp70: Anti-Apoptotic and Tumorigenic Protein

            Anne-Laure Rérole, Gaëtan Jego, and Carmen Garrido


17. Determination of Cell Survival or Death

            Alexander  E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai


18. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

            Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario


19. Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells

            Desheng Weng, Stuart K. Calderwood, and Jianlin Gong


20. Isolation of Heat Shock Protein Complexes

            Fernando D. Cuello-Carrión, Mariel A. Fanelli, Niubys Cayado-Gutiérrez, Gisela Castro, and Daniel R. Ciocca


21. Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein

            Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck


22. Investigating Receptors for Extracellular Heat Shock Proteins

            Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood


23. Analysis of Cellular Migration Using a Two-Chamber Methodology

            Michelle M. Kouspou and John T. Price

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