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Life Sciences - Biochemistry & Biophysics | Rat Genomics - Methods and Protocols

Rat Genomics

Methods and Protocols

Series: Methods in Molecular Biology, Vol. 597

Anegon, Ignacio (Ed.)

2010

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  • Comprehensive and an easily accessible reference volume for developing, running, and analyzing biomedical research using the rat as model system

Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems. The volume extensively covers topics including genome sequencing, quantitative trait loci mapping, and the identification of single nucleotide polymorphisms as well as the development of transgenic technologies such as nuclear cloning, lentiviral-mediated transgenesis, gene knock-down using RNA interference, gene knock-out by mutagenesis, and zinc finger nucleases plus exciting advances in the obtention of rat embryonic cell lines. As a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Comprehensive and up-to-date, Rat Genomics: Methods and Protocols thoroughly covers the current techniques used in labs around the world and overviews the applications of the data obtained, making it certain to be useful to the scientific community as a key source of references and methods.

Content Level » Research

Keywords » BAC - DNA - Microarray - PCR - Promoter - Quantitative PCR - Single Nucleotide Polymorphism - Translation - YAC - cell lines - cells - cryopreservation - gene expression - molecular biology - transgenic animals

Related subjects » Animal Sciences - Biochemistry & Biophysics - Human Physiology

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