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Life Sciences - Biochemistry & Biophysics | Matrix Metalloproteinase Protocols

Matrix Metalloproteinase Protocols

Series: Methods in Molecular Biology, Vol. 622

Clark, Ian M., Young, David A., Rowan, Andrew D. (Eds.)

2nd ed. 2010, XII, 472p.

A product of Humana Press
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  • Includes two comprehensive review chapters and twenty-five detailed method chapters
  • Provides state-of-the-art methodologies
  • Covers expression, purification, detection and assay of MMPs and TIMPs
  • Details many essential techniques in one easily-accessible volume
  • Is and updated and improved second edition
  • Chapters include "tricks of the trade" written by leading researchers

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms.  Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field.  Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Content Level » Research

Keywords » Escherichia coli - Microarray - Nucleotide - PCR - Proteomics - Single Nucleotide Polymorphism - enzymes - hybridization - molecular biology

Related subjects » Biochemistry & Biophysics

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