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Sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics
Shows how to perform two-dimensional electrophoresis with step-by-step instructions
Ensures successful results with tricks of the trade and troubleshooting tips
Offers methods that can be applied to a broad range of protein separation techniques
The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.
Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.
Sample Preparation and Fractionation: Volume 2 1 Application of fluorescence dye saturation labelling for differential proteome analysis of 1000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions (Kai Stühler) 2 Albumin and immunoglobulin depletion of human plasma (Rosalind E. Jenkins)
3 Multi-component Immunoaffinity Subtraction and Reversed-phase Chromatography of Human Serum (James Martosella) 4 Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies (Xiangming Fang) 5 Proteomics of Cerebrospinal Fluid: Methods for Sample Processing (John E. Hale) 6 Sample Preparation of Bronchoalveolar Lavage Fluid (Ruddy Wattiez) 7 Preparation of nasal secretions for proteome analysis (Begona Casado) 8 Preparation of Urine Samples for Proteomic Analysis (Rembert Pieper) 9 Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis (Qing-Yu He) 10 Sample Preparation of Culture Medium from Madine-Darby Canine Kidney Cells (Daniel Ambort) 11 Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin- Embedded Tissue (Timothy D. Veenstra) 12 Metalloproteomics in the Molecular Study of Cell Physiology and Disease (Hermann-Josef Thierse) 13 Protein extraction from green plant tissue (Ragnar Flengsrud) 14 The Terminator, a device for high-throughput extraction of plant material
(Bart van den Berg)
15 Isolation of mitochondria from plant cell culture (A. Harvey Millar) 16 Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants
17 Isolation of plant cell wall proteins (Rafael Pont-Lezica) 18 Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses (William J Simon) 19 Cell Wall Fractionation for Yeast and FungalProteomics (Concha Gil) 20 Collection of Proteins Secreted from Yeast Protoplasts in Active Cell Wall Regeneration (Concha Gil) 21 Sample preparation procedure for cellular fungi (Alois Harder) 22 ISOLATION AND ENRICHMENT OF SECRETED PROTEINS FROM FILAMENTOUS FUNGI (Wilson A. Francisco) 23 Isolation and Solubilization of Cellular Membrane Proteins from Bacteria (Kheir Zuobi-Hasona) 24 Isolation and Solubilization of Gram-Positive Bacterial Cell Wall-Associated Proteins (Mark J. Walker) 25 Cell fractionation of parasitic protozoa (Wanderley de Souza)