Ubiquitin-Proteasome Protocols

1. Front Matter

   Pages i-xi

   PDF

2. Biochemical Methods to Study the Ubiquitin-Proteasome System

   1. Front Matter

      Pages 1-1

      PDF

   2. Small-Molecule Inhibitors of Proteasome Activity

      • Maria Gaczynska, Pawel A. Osmulski

      Pages 1-22

   3. Purification of E1 and E1-Like Enzymes

      • Arthur L. Haas

      Pages 23-35

   4. Assays for RING Family Ubiquitin Ligases

      • Manabu Furukawa, Paul S. Andrews, Yue Xiong

      Pages 37-46

   5. Ubiquitin Chain Synthesis

      • Shahri Raasi, Cecile M. Pickart

      Pages 47-55

   6. Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast

      • David S. Leggett, Michael H. Glickman, Daniel Finley

      Pages 57-70

   7. Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome

      • Chang-Wei Liu, Elizabeth Strickland, George N. DeMartino, Philip J. Thomas

      Pages 71-81

   8. Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation

      • Chaim Kahana, Yuval Reiss

      Pages 83-96

   9. Assays of Proteasome-Dependent Cleavage Products

      • Stefan Tenzer, Hansjörg Schild

      Pages 97-115

   10. Identification of Components of Protein Complexes

       • Carol E. Parker, Maria R. Warren, David R. Loiselle, Nedyalka N. Dicheva, Cameron O. Scarlett, Christoph H. Borchers

       Pages 117-151

   11. Mass Spectrometric Determination of Protein Ubiquitination

       • Carol E. Parker, Viorel Mocanu, Maria R. Warren, Susanna F. Greer, Christoph H. Borchers

       Pages 153-173

   12. Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol

       • Robert J. Lee, Ardythe A. McCracken, Jeffrey L. Brodsky

       Pages 175-184

   13. Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation

       • Eric Carlson, Nathan Bays, Larry David, William R. Skach

       Pages 185-205

   14. Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization

       • Nathaniel S. Russell, Keith D. Wilkinson

       Pages 207-219

3. Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions

   1. Front Matter

      Pages 221-221

      PDF

   2. Measuring Ubiquitin Conjugation in Cells

      • Edward G. Mimnaugh, Leonard M. Neckers

      Pages 223-241

   3. Assays for Proteasome Assembly and Maturation

      • R. Jürgen Dohmen, Markus K. London, Christoph Glanemann, Paula C. Ramos

      Pages 243-254

   4. N-Terminal Ubiquitination

      • Aaron Ciechanover

      Pages 255-270

   5. Quantitating Defective Ribosome Products

      • Shu-Bing Qian, Jack R. Bennink, Jonathan W. Yewdell

      Pages 271-281

A collection of cutting-edge techniques for studying ubiquitin-dependent protein degradation via the proteasome. The topics covered range broadly from basic biochemistry to cellular assays to discovery techniques using mass spectrometric analysis. These biochemical and cellular methods are necessary to explore the ubiquitin-proteasome system and ubiquitin-proteasome-dependent functions. State-of-the-art and user-friendly, Ubiquitin-Proteasome Protocols offers novice and experienced bench scientists alike a thorough compendium of readily reproducible techniques that will accelerate discovery, enhance productivity, and permit manipulation of the system for varied research purposes.

• Endoplasmatisches Reticulum
• Termination
• biochemistry
• chemistry
• enzymes
• reproducible techniques

"...clearly written and thorough...a valuable addition to any lab entering, or already working in, the ubiquitin or proteasome field." - Doody's Health Science Book Review Journal

• Department od Medicine and the Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill

  Cam Patterson

• Department of Cell and Developmental Biology, University of North Carolona at Chapel Hill, Chapel Hill

  Douglas M. Cyr

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