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Written by experienced researchers from both the animal and plant fields
Contains some overviews and reviews to help guide understanding
Once believed to be involved mainly with energetics, including the production of ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years. In Redox-Mediated Signal Transduction: Methods and Protocols, experienced researchers with backgrounds in both the plant and animal sciences contribute timely methods and techniques that can be used to study this important aspect of biology. Beginning with an overview and methods for measuring compounds that affect redox and the redox state of cells, the book continues with reviews of the use of GFP and its derivatives, methods to study the impact of changing redox on proteins, and methods to study the exact molecular changes that may underlie the mechanisms of action of altering redox, among other subjects. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include step-by-step, readily reproducible protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Redox-Mediated Signal Transduction: Methods and Protocols is an ideal reference for those who wish to enter this exciting area of research as well as for those who simply wish for a more thorough understanding of the dramatic impact of redox in the control of cellular function.
Content Level »Research
Keywords »ATP production - Cellular function - Polyacrylamid-Gelelektrophorese - Redox biology - Signaling proteins - Termination - molecular biology - signal transduction
Chapter 1: The role of redox in signal transduction John T. Hancock Chapter 2: The measurement of nitric oxide and its metabolites in biological samples by ozone-based chemiluminescence. Andrew G. Pinder, Stephen C. Rogers, Afshin Khalatbari, Thomas E. Ingram, and Philip E. James. Chapter 3: Detection and measurement of reactive oxygen intermediates in mitochondria and cells Matthew Whiteman, Yuktee Dogra, Paul G. Winyard and Jeffrey S. Armstrong Chapter 4: Redox-Sensitive Green Fluorescent Protein: Probes for Dynamic Intracellular Redox Responses. A Review Mark B. Cannon and S. James Remington Chapter 5: Measuring redox changes in vivo in leaves: prospects and technical challenges Philip M. Mullineaux and Tracy Lawson Chapter 6: Imaging of intracellular hydrogen peroxide production with HyPer upon stimulation of HeLa cells with EGF Kseniya N. Markvicheva, Ekaterina A. Bogdanova, Dmitry B. Staroverov, Sergei Lukyanov, Vsevolod V. Belousov Chapter 7: Tools to investigate ROS sensitive signalling proteins Radhika Desikan, Steven J. Neill, Jenna Slinn and John T. Hancock Chapter 8: Methods for preparing crystals of reversibly oxidized proteins: crystallization of protein tyrosine phosphatase 1B as an example. Annette Salmeen and David Barford Chapter 9: Methods for the Study of Redox-Mediated Changes in p53 Structure and Function Kristine Mann Chapter 10: Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase. Susan M. Hutson, Leslie B. Poole, Steven Coles and Myra E. Conway Chapter 11: Detection of carbonylated proteins in 2-D SDS PAGE separations. Rukhsana Sultana, Shelley F. Newman, Quanzhen Huang, D. Allan Butterfield Chapter 12: Analysis of global and specific changes in the disulfide proteome using Redox 2D-PAGE Robert C. Cumming Chapter 13: Protein-thiol oxidation, from single proteins to proteome-wide analyses Natacha Le Moan, Frédérique Tacnet and Michel B. Toledano Chapter 14: Analysis of redox relationships in the plant cell cycle: determinations of ascorbate, glutathione and poly (ADPribose)polymerase (PARP) in plant cell cultures Christine H. Foyer, Till K. Pellny, Vittoria Locato and Laura De Gara Chapter 15: Generation and Detection of S-Nitrosothiols Christian Lindermayr, Simone Sell, and Jörg Durner