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2D PAGE: Sample Preparation and Fractionation

Volume 1

  • Book
  • © 2008

Overview

Editors:
  1. Anton Posch

    1. Bio-Rad Laboratories GmbH, Germany

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  • Contains step-by-step instructions that ensure successful results
  • Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls

Part of the book series: Methods in Molecular Biology (MIMB, volume 424)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xiii
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  3. Protein Labeling Techniques

    1. Front Matter

      Pages 71-71
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    2. Difference Gel Electrophoresis Based on Lys/Cys Tagging

      • Reiner Westermeier, Burghardt Scheibe
      Pages 73-85

    3. Isotope-Coded Two-Dimensional Maps: Tagging with Deuterated Acrylamide and 2-Vinylpyridine

      • Pier Giorgio Righetti, Roberto Sebastiano, Attilio Citterio
      Pages 87-99

    4. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

      • s Gruhler, Irina Kratchmarova
      Pages 101-111

    5. ICPL—Isotope-Coded Protein Label

      • Josef Kellermann
      Pages 113-123

    6. Radiolabeling for Two-Dimensional Gel Analysis

      • Hélian Boucherie, Aurélie Massoni, Christelle Monribot-Espagne
      Pages 125-135

  4. Fractionation of Proteins by Chemical Reagents and Chromatography

    1. Front Matter

      Pages 137-137
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    2. Sequential Extraction of Proteins by Chemical Reagents

      • Stuart J. Cordwell
      Pages 139-146

    3. Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg

      • Gert Van den Bergh, Lutgarde Arckens
      Pages 147-156

    4. Enriching Basic and Acidic Rat Brain Proteins with Ion Exchange Mini Spin Columns Before Two-Dimensional Gel Electrophoresis

      • Ning Liu, Aran Paulus
      Pages 157-166

    5. Reducing the Complexity of the Escherichia coli Proteome by Chromatography on Reactive Dye Columns

      • Phillip Cash, Ian R. Booth
      Pages 167-185

    6. Reducing Sample Complexity in Proteomics by Chromatofocusing with Simple Buffer Mixtures

      • Hong Shen, Xiang Li, Charles J Bieberich, Douglas D Frey
      Pages 187-203
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Keywords

About this book

This book, split into two volumes, presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, is still a fundamental research tool for nearly all protein-related scientific projects. The methods described here in detail are not limited to 2-DE and can also be applied to other protein separation techniques. Because each biological sample is unique, a suited sample preparation strategy has to consider the type of sample as well as the type of biological question being addressed. The complex nature of proteins often requires a multitude of sample preparation options. In addition, sample preparation is not only a prerequisite for a successful and reproducible Proteomics exp- iment, but also the key factor to meaningful data evaluation. Interestingly, not much attention was paid to this area during Proteomics methodology dev- opment and therefore this book is intended to explain in depth how proteins from various sources can be properly isolated and prepared for reproducible Proteome analysis.

Editors and Affiliations

  • Bio-Rad Laboratories GmbH, Germany

    Anton Posch

Bibliographic Information

  • Book Title: 2D PAGE: Sample Preparation and Fractionation

  • Book Subtitle: Volume 1

  • Editors: Anton Posch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-064-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-722-8Published: 19 March 2008

  • Softcover ISBN: 978-1-61737-742-6Published: 09 December 2010

  • eBook ISBN: 978-1-60327-064-9Published: 15 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 459

  • Number of Illustrations: 131 b/w illustrations, 2 illustrations in colour

  • Topics: Proteomics, Cell Biology, Biochemistry, general, Microbial Genetics and Genomics

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