Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes. This is done while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments.
Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology.
Microarray Technology, Volumes
Table of Contents
Volume 1 - Synthesis Methods
1. Introduction: Array Technology - An Overview
2. Current Microarray Surface Chemistries
David W. Grainger, Charles H. Greef, Ping Gong and Michael J. Lochhead
3. Non-Fouling Surfaces: A Review of Principles and Applications for Microarray Capture Assay Designs
Ping Gong and David W. Grainger
4. Optimization of Oligonucleotide DNA Microarray
Martin Dufva and Claus BV Christensen.
5. Detection of DNA Copy Number Alterations in Complex Genomes Using Array CGH
6. Evaluating the Quality of Data Microarray Measurements
Lili Wang, A.K. Gaigalas, M.B. Satterfield, M. Salit, Y. Zong, and J. Noble
7. Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents
Jyoti Choithani, B. Vaijayanthi, Pradeep Kumar, and Kailash Chand Gupta.
8. Choice of Polymer Matrix, its Functionalization and Estimation of Functional Group Density for Preparation of Biochips
Shweta Mahajan, B. Vaijayanthi , Gopal Rembhotkar, Kailash Chand Gupta, Pradeep Kumar.
9. Methods in High-Resolution, Array-Based Comparative Genomic Hybridization
Mark R. McCormick, Rebecca R. Selzer and Todd A. Richmond
10. Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression
Cheng-Chung and Konan Peck
11. In-Situ Oligonucleotide Arrays on Plastic
Jang B. Rampal , Peter J. Coassin and Robert S. Matson
12. Detecting Ligated Fragments on Oligonucleotide Microarrays - Optimizing Chip Design, Array MLPA Modification and Hybridization Parameters
Ian R Berry, Carol A. Delaney, Graham R. Taylor
13. Detection of SNPs in Cancer Related Genes by Minisequencing on a Microelectronic DNA Chip
Alexandre Ho-Pun-Cheung, Hafid Abaibou, Philippe Cleuziat and Evelyn Lopez- Crapez
14. Hybridization Analysis Using Oligonucleotide Probe Arrays
Robert S. Matson and Jang B. Rampal
15. In situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing
Bogdan V. Antohe and Patrick W. Cooley
16. Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays
Ming-Qun Xu, Inca Ghosh, Samvel Kochinyan and Luo Sun
17. Printing Low Density Protein Arrays in Microplates
Robert S. Matson Raymond C. Milton, Michael C. Cress and Jang B. Rampal
18. Protein Microarray Applications - Forward and Reverse Protein Arrays
Yaping Zong, Shanshan Zhang, Huang-Tsu Chen, Yunfei Zong, and Yaxian Shi.
19. Cell Microarray for Functional Exploration of Genomes
David Castel, Marie-Anne Debily, Amandine Pitaval and Xavier Gidrol
20. Quantification of Mixed-phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence
Kaisa Ketomaki and Harri Lonnberg.
21. Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays
Kazuo Yamamoto, Fumiko Yasukawa, and Seiichiro Ito
22. Nanotechnology: Moving from Microarrays Toward Nanoarrays
Hua Chen and Jun Li.