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Life Sciences - Biochemistry & Biophysics | Microarrays - Volume I: Synthesis Methods

Microarrays

Volume I: Synthesis Methods

Series: Methods in Molecular Biology, Vol. 381

Volume package: Microarrays

Rampal, Jang B. (Ed.)

Originally published by Academic Press, 2000

2nd ed. 2007

A product of Humana Press
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  • Meticulous exploration into state-of-the-art methods and protocols for microarray technology
  • Authors examine bioinformatics, an important aspect in analyzing large amounts of data that emerge during experiments

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes. This is done while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments.

Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology.

Microarray Technology, Volumes

Content Level » Research

Keywords » Biochip - DNA - Ligation - Microarray - Nucleotide - PAC - SNP - gene expression - genes - hybridization

Related subjects » Biochemistry & Biophysics - Biotechnology - Cell Biology - Human Genetics - Molecular Medicine

Table of contents 

Table of Contents Microarray Technology Volume 1 - Synthesis Methods 1. Introduction: Array Technology - An Overview Hartmut Seliger 2. Current Microarray Surface Chemistries David W. Grainger, Charles H. Greef, Ping Gong and Michael J. Lochhead 3. Non-Fouling Surfaces: A Review of Principles and Applications for Microarray Capture Assay Designs Ping Gong and David W. Grainger 4. Optimization of Oligonucleotide DNA Microarray Martin Dufva and Claus BV Christensen. 5. Detection of DNA Copy Number Alterations in Complex Genomes Using Array CGH Wei-Wen Cai 6. Evaluating the Quality of Data Microarray Measurements Lili Wang, A.K. Gaigalas, M.B. Satterfield, M. Salit, Y. Zong, and J. Noble 7. Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents Jyoti Choithani, B. Vaijayanthi, Pradeep Kumar, and Kailash Chand Gupta. 8. Choice of Polymer Matrix, its Functionalization and Estimation of Functional Group Density for Preparation of Biochips Shweta Mahajan, B. Vaijayanthi , Gopal Rembhotkar, Kailash Chand Gupta, Pradeep Kumar. 9. Methods in High-Resolution, Array-Based Comparative Genomic Hybridization Mark R. McCormick, Rebecca R. Selzer and Todd A. Richmond 10. Design and Fabrication of Spotted Long Oligonucleotide Microarrays for Gene Expression Cheng-Chung and Konan Peck 11. In-Situ Oligonucleotide Arrays on Plastic Jang B. Rampal , Peter J. Coassin and Robert S. Matson 12. Detecting Ligated Fragments on Oligonucleotide Microarrays - Optimizing Chip Design, Array MLPA Modification and Hybridization Parameters Ian R Berry, Carol A. Delaney, Graham R. Taylor 13. Detection of SNPs in Cancer Related Genes by Minisequencing on a Microelectronic DNA Chip Alexandre Ho-Pun-Cheung, Hafid Abaibou, Philippe Cleuziat and Evelyn Lopez- Crapez 14. Hybridization Analysis Using Oligonucleotide Probe Arrays Robert S. Matson and Jang B. Rampal 15. In situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing Bogdan V. Antohe and Patrick W. Cooley 16. Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays Ming-Qun Xu, Inca Ghosh, Samvel Kochinyan and Luo Sun 17. Printing Low Density Protein Arrays in Microplates Robert S. Matson Raymond C. Milton, Michael C. Cress and Jang B. Rampal 18. Protein Microarray Applications - Forward and Reverse Protein Arrays Yaping Zong, Shanshan Zhang, Huang-Tsu Chen, Yunfei Zong, and Yaxian Shi. 19. Cell Microarray for Functional Exploration of Genomes David Castel, Marie-Anne Debily, Amandine Pitaval and Xavier Gidrol 20. Quantification of Mixed-phase Hybridization on Polymer Microparticles by Europium (III) Ion Fluorescence Kaisa Ketomaki and Harri Lonnberg. 21. Measurement of the Sugar-Binding Specificity of Lectins Using Multiplexed Bead-Based Suspension Arrays Kazuo Yamamoto, Fumiko Yasukawa, and Seiichiro Ito 22. Nanotechnology: Moving from Microarrays Toward Nanoarrays Hua Chen and Jun Li.

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