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Therapeutic Proteins

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. C. Mark Smales

    1. Protein Science Group, Department of Biosciences, University of Kent, Canterbury, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. David C. James

    1. School of Engineering, University of Queensland, St. Lucia, Australia

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 308)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Biopharmaceutical Proteins

    • John R. Birch, Yemi Onakunle
    Pages 1-16

  3. Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli

    • Andrew G. Popplewell, Mukesh Sehdev, Mariangela Spitali, A. Neil C. Weir
    Pages 17-30

  4. Secretory Production of Therapeutic Proteins in Escherichia coli

    • Sang Yup Lee, Jong Hyun Choi, Sang Jun Lee
    Pages 31-41

  5. Expression of Recombinant LTB Protein in Marine vibrio VSP60

    • Wang Yili, Si Lusheng
    Pages 43-49

  6. Heterologous Gene Expression in Yeast

    • Lee J. Byrne, Kenneth J. O’Callaghan, Mick F. Tuite
    Pages 51-64

  7. Pharmaceutical Proteins From Methylotrophic Yeasts

    • Eric C. de Bruin, Erwin H. Duitman, Arjo L. de Boer, Marten Veenhuis, Ineke G. A. Bos, C. Erik Hack
    Pages 65-76

  8. Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems

    • Zheng Jin, Si Lusheng, Wang Yili
    Pages 77-86

  9. Large-Scale Transient Expression of Therapeutic Proteins in Mammalian Cells

    • Sabine Geisse, Martin Jordan, Florian M. Wurm
    Pages 87-98

  10. Site-Specific Integration for High-Level Protein Production in Mammalian Cells

    • Bhaskar Thyagarajan, Michele P. Calos
    Pages 99-106

  11. Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture

    • Lily Chu, Ilse Blumentals, Gargi Maheshwari
    Pages 107-121

  12. Transgene Control Engineering in Mammalian Cells

    • Beat P. Kramer, Martin Fussenegger
    Pages 123-143

  13. Fusion to Albumin as a Means to Slow the Clearance of Small Therapeutic Proteins Using the Pichia pastoris Expression System

    • William P. Sheffield, Teresa R. McCurdy, Varsha Bhakta
    Pages 145-154

  14. High-Throughput Recovery of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli

    • Amulya K. Panda
    Pages 155-161

  15. Isolation, Solubilization, Refolding, and Chromatographic Purification of Human Growth Hormone from Inclusion Bodies of Escherichia coli Cells

    • Surinder M. Singh, A. N. S. Eshwari, Lalit C. Garg, Amulya K. Panda
    Pages 163-176

  16. Large-Scale Preparation of Factor VIIa from Human Plasma

    • Teruhisa Nakashima, Kazuhiko Tomokiyo
    Pages 177-190

  17. Purification of Clinical-Grade Monoclonal Antibodies by Chromatographic Methods

    • Alberto L. Horenstein, Ilaria Durelli, Fabio Malavasi
    Pages 191-208

  18. Virus Elimination and Validation

    • Nicola Boschetti, Anna Johnston
    Pages 209-219

  19. Virus Removal by Nanofiltration

    • Marina Korneyeva, Scott Rosenthal
    Pages 221-231

  20. Determining Residual Host Cell Antigen Levels in Purified Recombinant Proteins by Slot Blot and Scanning Laser Densitometry

    • Aaron P. Miles, Daming Zhu, Allan Saul
    Pages 233-242
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Keywords

About this book

With the recent completion of the sequencing of the human genome, it is widely anticipated that the number of potential new protein drugs and targets will escalate at an even greater rate than that observed in recent years. However, identification of a potential target is only part of the process in developing these new next generation protein-based “drugs” that are increasingly being used to treat human disease. Once a potential protein drug has been identified, the next rate-limiting step on the road to development is the production of sufficient authentic material for testing, charact- ization, clinical trials, and so on. If a protein drug does actually make it through this lengthy and costly process, methodology that allows the production of the protein on a scale large enough to meet demand must be implemented. Furthermore, large-scale production must not compromise the authenticity of the final product. It is also nec- sary to have robust methods for the purification, characterization, viral inactivation and continued testing of the authenticity of the final protein product and to be able to formulate it in a manner that retains both its biological activity and lends itself to easy administration. Therapeutic Proteins: Methods and Protocols covers all aspects of protein drug production downstream of the discovery stage. This volume contains contributions from leaders in the field of therapeutic protein expression, purification, characterization, f- mulation, and viral inactivation.

Reviews

"...scientists in the area will find the material of value." - Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Protein Science Group, Department of Biosciences, University of Kent, Canterbury, UK

    C. Mark Smales

  • School of Engineering, University of Queensland, St. Lucia, Australia

    David C. James

Bibliographic Information

  • Book Title: Therapeutic Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: C. Mark Smales, David C. James

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599222

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-390-9Published: 15 August 2005

  • Softcover ISBN: 978-1-62703-848-5Published: 02 December 2014

  • eBook ISBN: 978-1-59259-922-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 482

  • Topics: Biochemistry, general

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