Skip to main content
SpringerLink
Log in

Chemical Biology

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Jonathan E. Hempel

    1. Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Charles H. Williams

    1. Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Charles C. Hong

    1. Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1263)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. In Vitro Chemical Genetic Screening

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Identification of Therapeutic Small-Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Readouts

      • Ozlem Kulak, Kiyoshi Yamaguchi, Lawrence Lum
      Pages 3-14

    3. Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease Alleles

      • Ari D. Brettman, Pauline H. Tan, Khoa Tran, Stanley Y. Shaw
      Pages 15-27

    4. Construction and Application of a Photo-Cross-Linked Chemical Array

      • Yasumitsu Kondoh, Kaori Honda, Hiroyuki Osada
      Pages 29-41

    5. High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells

      • Sean Spiering, Herman Davidovics, Paul J. Bushway, Mark Mercola, Erik Willems
      Pages 43-61

    6. Small-Molecule High-Throughput Screening Utilizing Xenopus Egg Extract

      • Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, Ethan Lee
      Pages 63-73

  3. In Vivo Chemical Genetic Screening

    1. Front Matter

      Pages 75-75
      Download chapter PDF

    2. Fission Yeast-Based High-Throughput Screens for PKA Pathway Inhibitors and Activators

      • Ana Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, Charles S. Hoffman
      Pages 77-91

    3. A Method for High-Throughput Analysis of Chronological Aging in Schizosaccharomyces pombe

      • Jessica Stephan, Ann E. Ehrenhofer-Murray
      Pages 93-101

    4. Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis

      • Manal J. Natto, Anthonius A. Eze, Harry P. de Koning
      Pages 103-110

    5. Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium

      • Thanh Theresa Dinh, Xuemei Chen
      Pages 111-125

    6. Small-Molecule Screening Using Drosophila Models of Human Neurological Disorders

      • Mickael Poidevin, Feiran Zhang, Peng Jin
      Pages 127-138

    7. High-Throughput Small-Molecule Screening in Caenorhabditis elegans

      • Sunitha Rangaraju, Gregory M. Solis, Michael Petrascheck
      Pages 139-155

    8. Whole-Organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish

      • Philipp Gut, Didier Y. R. Stainier
      Pages 157-165

    9. High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafish

      • Charles H. Williams, Charles C. Hong
      Pages 167-174

  4. Small Molecules in Chemical Biology

    1. Front Matter

      Pages 175-175
      Download chapter PDF

    2. Extraction Methods of Natural Products from Traditional Chinese Medicines

      • Jingyu Zhang, Jianying Han, Ayokunmi Oyeleye, Miaomiao Liu, Xueting Liu, Lixin Zhang
      Pages 177-185

    3. Bioassay-Guided Identification of Bioactive Molecules from Traditional Chinese Medicines

      • Jianying Han, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu et al.
      Pages 187-196

    4. NMR Screening in Fragment-Based Drug Design: A Practical Guide

      • Hai-Young Kim, Daniel F. Wyss
      Pages 197-208
Back to top

Keywords

About this book

This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential components of the chemical biology tool discovery process, the book is organizes into four parts that focus on platforms for molecular discovery in in vitro cellular systems, in vivo chemical genetic screening protocols, and methods used to discover functional protein targets. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and informative, Chemical Biology: Methods and Protocols seeks to improve the success rate of the chemical biology field through the dissemination of detailed and experiential knowledge.

Editors and Affiliations

  • Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, USA

    Jonathan E. Hempel

  • Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, USA

    Charles H. Williams

  • Department of Medicine - Cardiovascular Medicine, Department of Pharmacology, Department of Cell and Developmental Biology, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, USA

    Charles C. Hong

Bibliographic Information

  • Book Title: Chemical Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Jonathan E. Hempel, Charles H. Williams, Charles C. Hong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2269-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2268-0Published: 24 January 2015

  • Softcover ISBN: 978-1-4939-5284-7Published: 24 September 2016

  • eBook ISBN: 978-1-4939-2269-7Published: 24 January 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 333

  • Number of Illustrations: 25 b/w illustrations, 40 illustrations in colour

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation